The Effect of Smoking and Non-surgical Periodontal Treatment on Gingival Crevicular Fluid Cyclophilin A and EMMPRIN Levels

This study aimed to investigate the effect of smoking and non-surgical periodontal treatment on gingival crevicular fluid CyPA and EMMPRIN levels in patients with periodontitis. Twenty-eight patients with periodontitis (14 non-smoker and 14 smoker) and 30 control subjects (15 non-smoker and 15 smoker) were included into the study. Non-surgical periodontal treatment was applied to all periodontitis patients. Periodontal parameters and GCF CyPA and EMMPRIN levels were evaluated during 3 months following non-surgical periodontal treatment. GCF CyPA and EMMPRIN levels were analyzed by ELISA.

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Key information

Conditions

Smoking Behavior Mouth Diseases Non-surgical Periodontal Treatment Periodontal Diseases Periodontitis Stomatognathic Diseases

Age range

29 year–61 year

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

generalized periodontitis with stage III-IV & Grade B
no systemic and periodontal diseases
smokers (minimum 10 cigarettes per day for at least ten years)
non-smokers (never smoked)

Exclusion criteria

Systemic diseases
Infectious diseases
Pregnancy or lactating
Having periodontal treatment during the last 6 months
Having antibiotic treatment within the past 3 months
Former smokers

Treatment and study plan

Non-surgical periodontal treatment

Procedure

Non-surgical periodontal treatment program was as following: First, motivation and oral hygiene instructions were given to all participants with periodontitis. The modified-Bass tooth brushing technique with a given dentifrice and toothbrush twice a day, and dental flossing/ interdental brushing once a day was advised to all patients with periodontitis. Second, scaling using an ultrasonic device and root planning by hand instruments under local anesthesia was performed.

Primary outcomes

Change in gingival crevicular fluid CypA total amounts

Time frame: Baseline, 1 week, 1., 3. months

GCF CyPA levels were analyzed by ELISA
Change in gingival crevicular fluid EMMPRIN total amounts

Time frame: Baseline, 1 week, 1., 3. months

GCF EMMPRIN levels were analyzed by ELISA

Secondary outcomes

Change in probing depth

Time frame: Baseline, 1., 3. months

Probing depth was measured with Williams periodontal probe.
Change in bleeding on probing

Time frame: Baseline, 1., 3. months

Bleeding on probing was evaluated the presence or absense following bleeding upon probing.

Sponsors and collaborators

Lead sponsor

Ege University

Other

Registry information

Important dates

Study start: 2014
Primary completion: 2015
Study completion: 2015
First posted: Jun 12, 2020
Registry last updated: Jun 12, 2020

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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Completed