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Completed

NCT Number: NCT04321772

Muscle Response to High Intensity Interval Resistance Training (HIIRT)

Resistance training (RT) is one of the most important stimuli for muscle hypertrophy and it plays an important role on weight loss and fatty acid oxidation increase. RT affects anabolic pathways but the differences among various training techniques has been till now were poorly investigated. The aim of this study was to compare the effect of two different intensity of training, high-intensity interval resistance training (HIIRT) and traditional resistance training (TRT), on muscle signalling pathway.

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Key information

Conditions

Exercise Behavior Motor Activity Resistance Training

Age range

20 year–30 year

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

Primary location

Nutrition and Exercise Lab, DSB, University of Padova

Padova, 35131, Italy

About this study

Nine young healthy subjects performed HIIRT and TRT protocol in two different moments and with different legs. HIIRT technique consisted of three sets of: 6 repetitions at 6RM (6 repetitions maximum) and then 20 seconds of rest and 2/3 repetitions (until exhaustion) repeated for 3 times with 2'30" rest between sets; while TRT consisted of 3 sets of 15 reps with 75 sec of rest between sets. Biopsies from the vastus lateralis were taken at baseline (pre), immediately (0h) at the end of training, 6 hours (6h) and 24 hours (24h) after training. Western blot and RT-PCR mRNA (Reverse transcriptase-polymerase chain reaction) analysis were performed to assess muscle signalling pathway activation

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • moderate active
  • experience with resistance training

Exclusion criteria

  • history of recent myocardial infarction,
  • severe cardiac arrhythmia,
  • unstable angina,
  • poorly controlled hypertension,
  • poorly controlled diabetes mellitus,
  • frequent or complex ventricular ectopy which might interfere with one's ability to adhere to exercise protocols

Treatment and study plan

High Intensity Interval Resistance Training

Other

Subjects trained one leg with HIIRT

Traditional Resistance Training

Other

Subjects trained the other leg with HIIRT

Primary outcomes

  1. pAMPKThr172 in skeletal muscle tissue measured by immunoblot

    Time frame: 24 hours

    measurement of pAMPKThr172 in skeletal muscle tissue taken by biopsy

  2. pERK 1/2Thr202/Tyr204 in skeletal muscle tissue measured by immunoblot

    Time frame: 24 hours

    measurement of pERK 1/2Thr202/Tyr204 in skeletal muscle tissue taken by biopsy

  3. pS6Ser235/236 in skeletal muscle tissue measured by immunoblot

    Time frame: 24 hours

    measurement of pS6Ser235/236 in skeletal muscle tissue taken by biopsy

  4. p4EBP1Thr37/46 in skeletal muscle tissue measured by immunoblot

    Time frame: 24 hours

    measurement of p4EBP1Thr37/46 in skeletal muscle tissue taken by biopsy

  5. ACC (acetyl-coenzyme A carboxylase) phosphorylation in skeletal muscle tissue measured by immunoblot

    Time frame: 24 hours

    ACC phosphorylation in skeletal muscle tissue taken by biopsy

  6. IGF-1 mRNA (messenger ribonucleic acid) in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

    Time frame: 24 hours

    IGF-1 mRNA in skeletal muscle tissue taken by biopsy

  7. IGF-1ea (insulin like growth factor) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

    Time frame: 24 hoursn

    IGF-1ea mRNA in skeletal muscle tissue taken by biopsy

  8. MGF (mechanical growth factor) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

    Time frame: 24 hours

    MGF mRNA in skeletal muscle tissue taken by biopsy

  9. STARS mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

    Time frame: 24 hoursn

    STARS mRNA in skeletal muscle tissue taken by biopsy

  10. Myogenin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

    Time frame: 24 hours

    Myogenin mRNA in skeletal muscle tissue taken by biopsy

  11. IL-6 (interleukin 6) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

    Time frame: 24 hours

    IL-6 mRNA in skeletal muscle tissue taken by biopsy

  12. PGC1a4 (Peroxisome proliferator-activated receptor gamma coactivator 1-alpha 4) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

    Time frame: 24 hours

    PGC1a4 mRNA in skeletal muscle tissue taken by biopsy

  13. PGC1a (Peroxisome proliferator-activated receptor gamma coactivator 1-alpha) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

    Time frame: 24 hours

    PGC1a mRNA in skeletal muscle tissue taken by biopsy

  14. Atrogin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

    Time frame: 24 hours

    Atrogin mRNA in skeletal muscle tissue taken by biopsy

  15. Beclin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

    Time frame: 24 hours

    Beclin mRNA in skeletal muscle tissue taken by biopsy

  16. Myostatin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

    Time frame: 24 hours

    Myostatin mRNA in skeletal muscle tissue taken by biopsy

Sponsors and collaborators

Lead sponsor

University of Padova

Other

Collaborators

  • University of Roma La Sapienza

Registry information

Official study title

Effects of Exercise Intensity and Rest Intervals on Intracellular Signals and Anabolic Response of Skeletal Muscle to Resistance Training

Acronym: HIIRT

Important dates

Study start
2012
Primary completion
2012
Study completion
2013
First posted
Mar 25, 2020
Registry last updated
Mar 25, 2020

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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