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OpenTrials
Completed

NCT Number: NCT05835401

Effects of Dietary Nitrate Intake on Cardiovascular Performance in Response to Hand Grip Test in Bodybuilders

Through a randomized, crossover, double-blind, placebo-controlled clinical trial, 20 bodybuilders will participate a acute-day trial with two intervention protocols: 1) placebo and 2) nitrate; in which will ingest beet juice with or without NO3 in its composition with a 7-day washout interval.

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Key information

Conditions

Steroid Abuse Cardiovascular Abnormalities Cardiovascular Diseases Congenital Abnormalities Congenital, Hereditary, and Neonatal Diseases and Abnormalities

Age range

18 year–45 year

Sex eligibility

Male

Study type

Interventional

Phase

Not applicable

Primary location

EEFERP - USP / Laboratório de Fisiologia do Exercício e Metabolismo

Ribeirão Preto, São Paulo, Brazil

Who can participate

Healthy volunteers accepted: No

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Anabolic steroid use in the past or actually;
  • Participation at least one bodybuilding competition;

Exclusion criteria

  • Previous history of acute myocardial infarction and/or stroke;
  • Allergy or intolerance to nitrate, gluten, or milk;
  • Medications (proton pump inhibitors, beta-blockers, calcium channels antagonists).

Treatment and study plan

Beetroot juice rich in nitrate

Dietary Supplement

The participant will intake beetroot juice rich in NO3 (800mg). Two hours after intake the last bottle of juice, the participants will go to the laboratory for the handgrip and cardiovascular tests evaluation. Before crossover protocol a 7-day washout interval will be given for the purification of the compounds provided by the protocols.

Beetroot juice depleted in nitrate

Dietary Supplement

The participant will intake beetroot juice depleted in NO3. Two hours after intake the last bottle of juice, the participants will go to the laboratory for the handgrip and cardiovascular tests evaluation. Before crossover protocol a 7-day washout interval will be given for the purification of the compounds provided by the protocols.

Primary outcomes

  1. 1. Values of systolic and diastolic blood pressure (mmHg)

    Time frame: up to 2 years

Secondary outcomes

  1. Plasmatic concentrations of nitrate and nitrite (mmol/L)

    Time frame: up to 2 years

  2. Values of heart rate variability (ms)

    Time frame: up to 2 years

  3. Macrovascular function (Flow mediated dilatation%)

    Time frame: up to 2 years

  4. Values of heart rate (beats per minute)

    Time frame: up to 2 years

  5. Values of hand grip strength (kgF)

    Time frame: up to 2 years

Sponsors and collaborators

Lead sponsor

University of Sao Paulo

Other

Registry information

Important dates

Study start
2023
Primary completion
2023
Study completion
2023
First posted
Apr 28, 2023
Registry last updated
Jul 28, 2025

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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