Assessment of Rheological Parameters of Human Sputum.

This study includes two visits to 48 hours apart.

During the first visit :

• Patients with COPD or cystic fibrosis will have a spontaneous expectoration
• Then all study participants will have an induced expectoration with hypertonic salin solution (4.5%).

During the second visit :

• Patients with COPD or cystic fibrosis will have a spontaneous expectoration
• Then, patients with COPD, asthma and healthy volunteers will have an induced expectoration with hypertonic salin solution (4.5%). Patients with cystic fibrosis will have a spontaneous expectoration 1 hour after an RhDNAse nebulization.

A control spirometry will be perform before each expectoration and during induced expectoration if necessary.

All sputum sample collected will be homogenized and then divided into two equal volumes in order to perform two separate rheological measurements of 15 minutes.

Inclusion criteria

of healthy volunteers:

• 18>BMI>29
• No smoker
• No acute disease in the previous month

Exclusion criteria

of healthy volunteers:

• asthma, COPD, cystic fibrosis
• contraindications for spirometry
• pregnant woman
• legal exclusion criteria

Inclusion criteria

of patients with cystic fibrosis:

• cystic fibrosis with bronchial disorder. Diagnosis confirmed by a reference center

Exclusion criteria

of patients with cystic fibrosis:

• Forced Expiratory Volume in 1 second (FEV1 ) ≤ 40%, contraindications for RhDNAse, patient with chronic impairment of lung function (PaO2<60 mmHg at rest). Patient who is unable to perform a spontaneous expectoration.
• case of acute exacerbation during the last month
• contraindications for spirometry
• pregnant woman
• legal exclusion criteria

Inclusion criteria

of patients with COPD:

• COPD with FEV1/Forced Vital Capacity (FVC) <70% after administration of a beta-2 agonist

Exclusion criteria

of patients with COPD:

• Patient with chronic impairment of lung function and/or FEV1≤ 40%. Patient who is unable to perform a spontaneous expectoration.
• case of acute exacerbation during the last month
• contraindications for spirometry
• pregnant woman
• legal exclusion criteria

Inclusion criteria

of patients with asthma:

• diagnosis of asthma confirmed by a Pulmonologist

Exclusion criteria

of patients with COPD:

• Patient with chronic impairment of lung function and/or FEV1≤ 50%.
• Very unstable Asthma
• case of acute exacerbation during the last month
• contraindications for spirometry
• pregnant woman
• legal exclusion criteria