Zynamite® in Cognition and Mood in University Students

Primary outcomes

1. del POMS (Profile Of Mood States)

   Time frame: Up to fourteen days

   It has a total of 6 subscales: tension (score range, 0-36), depression (score range, 0-60), anger (score range, 0-48), fatigue (score range, 0-28), vigor (score range, 0-32), and confusion (score range, 0-28), and a total mood disturbance (score range, -32 to 200). Each of the questions will be scored from 0 (not at all) to 4 (very much so). The total mood disturbance score is calculated by summing the five negative subscale scores (tension, depression, anger, fatigue, and confusion) and subtracting the vigor score. Higher scores for the total mood disturbance score indicate a greater degree of mood disturbance.

2. Rey Auditory Verbal Learning Test (RAVLT)

   Time frame: Up to fourteen days

   The Rey Auditory Verbal Learning Test (RAVLT) is a neuropsychological test used to assess short- and long-term memory. The structure of the RAVLT consists of several parts:

   • Learning: Participants are read a list of 15 unrelated words (List A) in five consecutive trials.
   • Delayed Recall: The participant is asked to recall and repeat back the words from List A. To establish the score for this test, the following elements are considered to calculate the scores:

   • Total learning score: The total number of words correctly recalled in the five trials of List A is added up. Normal scores are 6 words correctly recalled in the first trial and 12 or 13 in the fifth trial. This reflects the ability for learning and short-term memory.
   • Delayed Recall: The number of words recalled from List A after the time interval without practice (20-30 minutes) is noted. This assesses long-term memory. The score can range from 0 to 15 points.

3. Trail Making Test (TMT)

   Time frame: Up to fourteen days

   It is used to assess executive function and specifically measures timed motor and visual tasks. This test is divided into two parts: Part A (TMT-A), which assesses psychomotor attention and speed and consists of connecting consecutively numbered circles; and Part B (TMT-B), which is based on connecting alternating circles of letters and numbers and measures executive function. A longer time spent completing the test is interpreted as worse performance.

4. The Digit Symbol Substitution Test (DSST)

   Time frame: Up to fourteen days

   Is a paper-and-pencil cognitive test presented on a single sheet of paper that requires the subject to match symbols to numbers according to a key located at the top of the page. The subject copies the symbol into spaces below a row of numbers. Scoring is based on the number of correct symbols the participant manages to substitute in 90 seconds of time.

5. Stroop Color and Word Test

   Time frame: Up to fourteen days

   It is used to assess selective attention. This test has a total of 3 slides, each lasting 45 seconds, with a total time of 5 minutes to complete the entire test. In order to read each slide correctly, it must be read from top to bottom and from left to right. The first slide (Stroop Word) is made up of 100 words in which the words "RED", "BLUE" and "GREEN" are repeated, ordered randomly and printed in black ink. This part assesses the number of words read in 45 seconds. After this time, participants must circle the last word they have read. The second slide (Stroop Color) is made up of 100 identical elements "XXXX" in blue, red and green ink. The participant must name the colour of "XXXX" as quickly as possible. Finally, the third slide (Stroop Word-Color) consists of 100 written words that refer to a colour, but are printed in a different colour. Participants must say the color of the ink each word is written in. Higher scores indicate better performance.