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OpenTrials
Completed

NCT Number: NCT02884063

Utilizing Free DNA in Embryo Culture for PGT

In the way for developing and optimizing protocol to be used as non- invasive methodology used as routine testing for PGS.

This protocol is to be adapted to replace the using of life embryo cells for genetic testing and aneuploidy study as well as for any type of genetic testing including single gene disorder or HLA typing or study.

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Key information

Conditions

Chromosome Abnormalities Chromosome Aberrations Congenital, Hereditary, and Neonatal Diseases and Abnormalities Genetic Diseases, Inborn Mutation Pathologic Processes Pathological Conditions, Signs and Symptoms

Age range

20 year–45 year

Sex eligibility

All sexes

Study type

Observational

About this study

Compare 100 embryos studied using NGS for chromosomal aneuploidies, and compare the results came from free media DNA with this results.

Who can participate

Healthy volunteers accepted: No

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • all abnormal/aneuploidy embryos rejected for embryo transfer.

Exclusion criteria

  • all embryos had unsuccessful diagnosis with blastomere biopsy.

Treatment and study plan

NGS reading for Media Free DNA

Procedure

NGS reading for Media Free DNA instead of blstomer biopsy

Primary outcomes

  1. DNA isolation

    Time frame: 2 years

    reading amplified DNA using NGS

Secondary outcomes

  1. NGS evaluation

    Time frame: 2 years

    Free DNA isolation and amplification using WGA

  2. Comparison with cell reading using NGS

    Time frame: 2 years

    compare the obtained result with the results provided collected for the same embryo using its blastomer for PGS

Sponsors and collaborators

Lead sponsor

Al Baraka Fertility Hospital

Other

Collaborators

  • Ibn Sina Hospital
  • Sadat City University

Registry information

Official study title

Utilizing Free DNA in Embryo Culture Media for Preimplantation Genetic Screening

Important dates

Study start
2015
Primary completion
2018
Study completion
2019
First posted
Aug 30, 2016
Registry last updated
Aug 19, 2021

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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