Treatment of Intrabony Defects With L-PRF Membrane With or Without Collagen Membranes

• Patients having 2-3 osseous bony wall infrabony periodontal lesions are enrolled in the present study. They have to be free from any systemic disease that contraindicates periodontal surgeries.
• Baseline data are obtained including the GI, PI, PD, CAL and preoperative paralleling periapical radiographs.
• Full thickness mucoperiosteal flaps are elevated and thorough debridement of the periodontal bony defect is performed.
• for OFD group; following debridement and saline irrigation, wound closure proceeds.
• for the L-PRF group; L-PRF is prepared and applied within the defect before primary closure.
• for the L-PRF + CM: similar procedure like the second group is performed but with an additional protective layer of collagen membrane on top of the defect before wound closure.
• from the second day postoperative; Periopaper strips are used to obtain a crevicular fluid sample from the sulcus
• At 6 months postoperative: the same baseline clinical measurements are obtained.

Inclusion criteria

• Minimum of one interproximal pocket probing depth of ≥6 mm and/or ≥5 mm CAL after 4 weeks from phase I execution
• 2 or 3 osseous wall interproximal intrabony defects that are ≥3mm in depth

Exclusion criteria

• Systemic diseases or conditions that contraindicate periodontal surgeries and/or affect the formed elements of the blood
• Patients who received antibiotic therapy and/or anti-inflammatory drug within the past 6 months
• Vulnerable groups
• Interdental craters and 1 wall osseous defects
• Active periapical pathosis