the Treatment of Human Bone Marrow Mesenchymal Stem Cells in Ocular Corneal Burn

Corneal burn is a ocular damage disease included chemically burned and thermally burned. Surgery of corneal transplantation，amniotic membrane transplantation are some of effective,however,these therapy are expensive and the transplantation resources are limited. To arrest the inflammatory phase, several types of immunosuppressive treatments have been investigated. Corticosteroids also is important, however, long time usage of corticosteroids often cause severe side-effects. Human bone marrow mesenchymal stem cells (MSC) have the biological characteristics of self -renewal, immune regulation, multidirectional differentiation and tissue repair. Our preliminary research showed that in corneal alkali injury rats, the MSC can accelerated the cornea repair, inhibited angiogenesis. Many animal research also revealed that MSC have effect on the ocular alkali burned. And subconjunctivity injection is efficient, the clinical study of MSC on treating other disease have been developed rapidly recently, in further ,the outcome are encouraging, and no side-effect related MSC was reported, MSC can come from bone marrow, Umbilical cord blood，Adipose tissue and so on, but bone marrow MSC is mostly common used. The investigators propose to assess the efficacy and safety of human bone marrow mesenchymal stem cell in the treatment of corneal burn in human.

Inclusion criteria

• must be ocular burns including chemically burned or the thermally burned
• the severity degree should above the Ⅳ degree,including the Ⅳ degree(according the classification of Dua standard,2001)
• the subjects are willing to accept this research,and promise to coordinate with the researchers during the follow up period
• the subjects should abide by the laws and rules of the study.
• the incident time should be within 2 weeks -

Exclusion criteria

• the visual acuity is blind in any of the eye
• have corneal perforation or have the corneal perforation tendency
• have been accepted surgury on eyeball after trauma
• IOP≥25mmHg even after antiglaucoma
• have the history of other corneal diseaze or surgury
• have the history of radiotherapy or surgury in the eyeball
• associated with corneal ulcer or endoophthalmitis
• uncontrolled hypertension(≥150/95mmHg)
• abnormal liver and renal function
• the pregancy women -