The Function and Proteome Alteration of HDL Subclass After Aerobic Exercise

The aim of present study is to test the effects of 12-week moderate aerobic exercise on the proteome and function alteration of HDL sub-fractions in male youth with abdominal obesity.

Study participants: youth males with abdominal obesity between the ages of 18 and 30 years were eligible for participation. Exclusion criteria were cardiovascular disease, diabetes or other chronic diseases, treatment with any medications, and had surgical procedures within the last 6 months. This study was approved by the Medical Ethics Committee at the Sun Yat-sen Memorial Hospital, complies with the Declaration of Helsinki principles and written informed consent would be obtained from all participants.

Data collection: anthropometric measurements, physical examination and blood samples were collected at 0 and 12 weeks. Cardiorespiratory measurements of each participant was measured using a bicycle-based graded exercise test at baseline.

Intervention: the exercise intervention consisted of 12 weeks of aerobic exercise training of moderate intensity (about 75 percentage of peak heart rate) of 40-60 min per time, 3-5 times per week, at least 150 min per week. All participants were instructed to maintain their usual dietary intakes. Completing a minimum of 80 percentage training sessions was satisfactory.

HDL isolation: HDL particles were fractionated into small, medium and large (S/M/L)-HDL subfractions by an ÄKTA fast protein liquid chromatography (FPLC) system.

Proteomics Experiments: equal volumes of L/M/S-HDL subclass were applied to the CSH resin, trypsinized, and then recognized via LC-MS. HDL proteins in HDL subclass were detected by MS-based label-free quantitation

HDL functions: cholesterol efflux capacity, anti-inflammation and anti-oxidative capacity, endothelial protective function

Analysis: data are expressed as mean and SD for continuous variables, or percentage for category variables. Difference of variables between baseline and after exercise were compared using analysis of Paired t-test or Mann Whitney U test. P<0.05 was considered statistically significant.

Inclusion criteria

• Inactive youth males with abdominal obesity: BMI>28 (weight in kilograms divided by height in meters squared) and weight stable for 6 months, waist to height ratio was ≥0.5 and/or waist circumference was ≥102 centimetres between the ages of 18 and 30 years.

Exclusion criteria

• Participants with cardiovascular disease, diabetes or other chronic diseases, treatment with any medications, and had surgical procedures within the last 6 months.