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Completed

NCT Number: NCT07048912

The Effect of Acute Beetroot Juice Supplementation on Anaerobic Performance in Football Players

The aim of this clinical trial is to examine the effects of two different natural fruit-vegetable blend beverages on short-duration, high-intensity exercise performance and muscle oxygenation in young male football players. The study also investigates how these beverages influence certain metabolic responses. The main questions it seeks to answer are:

* Does one of the beverages improve peak and mean power output during the Wingate test? * How do these beverages affect muscle oxygenation and post-exercise recovery? * Researchers will compare the effects of the two beverages on performance and metabolic markers.

Participants:

* Consume one of the two natural beverages 2.5 hours before testing (assigned at random) * Perform a 30-second high-intensity Wingate cycling test * Undergo measurements of muscle oxygenation, heart rate, blood pressure, blood lactate, and blood glucose during and after exercise * Maintain consistent dietary and physical activity routines and avoid certain foods prior to testing

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Key information

Conditions

Exercise Performance Supplementation

Age range

18 year–19 year

Sex eligibility

Male

Study type

Interventional

Phase

Not applicable

Primary location

Sakarya University of Applied Sciences, Faculty of Sport Sciences, Exercise and Sports Sciences Education, Application and Research Centre

Sakarya, Turkey (Türkiye)

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Healthy males over 18 years old
  • Performing resistance exercise at least three times per week
  • At least 5 years of football training experience

Exclusion criteria

  • Contraindications to Wingate exercise
  • Presence of cardiometabolic disease
  • Current use of ergogenic supplementation
  • Smokers

Treatment and study plan

Beetroot Juice (BJ)

Dietary Supplement

This intervention involves a single 140 mL dose of a commercially available concentrated beetroot juice supplement (Beet-It Pro Elite Shot, James White Drinks Ltd., UK), containing approximately 12.8 mmol (800 mg) of dietary nitrate. Participants consume the juice 2.5 hours prior to performing a 30-second Wingate anaerobic test. The purpose of this intervention is to examine its acute effects on anaerobic performance, muscle oxygenation, and metabolic responses.

Blackcurrant Juice (Placebo)

Dietary Supplement

This intervention involves a single 140 mL dose of a low-calorie blackcurrant juice (Pfanner, Austria) with negligible nitrate content (<0.1 mmol). The placebo is matched to beetroot juice in appearance, taste, and packaging to ensure double-blind conditions. It is consumed 2.5 hours before the Wingate test and serves as the control condition.

Primary outcomes

  1. Mean power output during the 30-second Wingate anaerobic test

    Time frame: Day 1 (2.5 hours after supplementation, during Wingate test)

    Average power (in watts) generated during the 30-second Wingate cycling test will be compared between beetroot juice and placebo conditions to assess anaerobic performance.

Secondary outcomes

  1. Muscle oxygen saturation (SmO₂)

    Time frame: Day 1 (pre-, during, and post-exercise on the day of Wingate test)

    SmO₂ will be assessed using Near-Infrared Spectroscopy (NIRS) on the vastus lateralis muscle. Measurements will be taken continuously before, during, and after the 30-second Wingate anaerobic test to evaluate changes in muscle oxygenation in response to the intervention.

Sponsors and collaborators

Lead sponsor

Melike Nur Eroğlu

Other

Registry information

Important dates

Study start
2024
Primary completion
2024
Study completion
2024
First posted
Jul 3, 2025
Registry last updated
Jul 3, 2025

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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