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Recruiting

NCT Number: NCT06188767

Testosterone-induced Muscle Memory and Underlying Mechanisms

The purpose of this project is to investigate underlying mechanisms of testosterone- and resistance training-induced muscle hypertrophy and muscle memory in humans.

Recruiting

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Key information

Conditions

Skeletal Muscle Physiology

Age range

18 year–40 year

Sex eligibility

Male

Study type

Interventional

Phase

Not applicable

Primary location

The August Krogh Section for Human and Molecular Physiology, Department of Nutrition, Exercise and Sports, University of Copenhagen

Copenhagen, 2100, Denmark

Location status: Recruiting

Location contact

Lukas Moesgaard, MSc

CONTACT

[email protected]

+4561724440

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Healthy men and women
  • BMI of <26 and normal ECG and blood pressure

Exclusion criteria

  • Smoking
  • Chronic disease,
  • Use of prescription medication
  • Pain due to current or previous musculoskeletal injury
  • Resistance training more than once per week in the 12 months leading up to the intervention
  • Current or previous use of prohibited anabolic substances

Treatment and study plan

Testosterone Undecanoate

Drug

Following testosterone/placebo injection, participants undergo supervised resistance training for 4 weeks. This period is followed by a washout period with no drug and either continued resistance training or detraining for 16 weeks. Thereafter, another 4-week period with supervised resistance training is commenced, with no drug.

Primary outcomes

  1. Fiber cross-sectional area

    Time frame: The change in fiber cross-sectional is assessed at baseline, 4, 20 and 24 weeks

    Immunohistochemistry to determine fiber cross-sectional area

  2. Myonuclei

    Time frame: The change in myonuclei is assessed at baseline, 4, 20 and 24 weeks

    Immunohistochemistry to determine number of myonuclei

  3. Skeletal muscle proteome

    Time frame: The change in the muscle proteome is assessed at baseline, 4, 20 and 24 weeks

    Pathway enrichment analysis of the muscle proteome using mass spectrometry-based proteomics

Secondary outcomes

  1. Lean mass

    Time frame: The change in lean mass is assessed at baseline, 4, 20 and 24 weeks

    Lean mass assessed by dual X-ray absorptiometry (DXA)

  2. Satellite cells

    Time frame: The change in satellite cells is assessed at baseline, 4, 20 and 24 weeks

    Immunohistochemistry to determine number of satellite cells

  3. Muscle strength

    Time frame: The change in 1-repetition maximum is assessed at baseline, 4, 20 and 24 weeks

    1-repetition maximum

Study contacts

Contact information is provided by the study sponsor or research team.

Lukas Moesgaard

CONTACT

[email protected]

+4561724440

Sponsors and collaborators

Lead sponsor

Morten Hostrup, PhD

Other

Registry information

Official study title

Long-term Effects of Anabolic Androgenic Steroids on Muscle Growth Potential: Testosterone-induced Muscle Memory and Underlying Mechanisms

Important dates

Study start
2025
Primary completion
2027
Study completion
2028
First posted
Jan 3, 2024
Registry last updated
Apr 10, 2025

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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