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OpenTrials
Completed

NCT Number: NCT04136457

Proteome Remodelling

The purpose of the project is to investigate the proteome signature dynamics and remodelling in human skeletal muscle induced by exercise training

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Key information

Conditions

Skeletal Muscle Physiology

Age range

18 year–45 year

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

Primary location

August Krogh Building

Copenhagen, Denmark

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Healthy men and women
  • VO2max > 55 mL/min/kgLM for men and > 50 for women (only applicable for endurance and sprint training interventions)
  • BMI < 26

Exclusion criteria

  • Chronic disease deemed by the medical doctor to pose a risk or to affect study outcomes
  • smoking
  • Use of prescription medicine deemed by the medical to affect study outcomes
  • Pregnancy

Treatment and study plan

Endurance training

Other

Subjects perform a period of endurance training

Resistance training

Other

Subjects perform a period of resistance training

sprint training

Other

Subjects perform a period of sprint training

Primary outcomes

  1. Skeletal muscle proteome signature

    Time frame: Change from baseline at week 6 or 8 depending on the intervention

    Pathway enrichment analysis of the muscle proteome

  2. Anabolic signaling

    Time frame: Change in anabolic signaling from rest to 1 hour following resistance exercise both at baseline and following 8 weeks of resistance training

    Western blotting of proteins related to anabolic signaling

Secondary outcomes

  1. Skeletal muscle mitochondrial respiration

    Time frame: Change from baseline at week 6 following endurance and sprint training

    High resolution respirometry

  2. Maximal oxygen consumption

    Time frame: Change from baseline at week 6 following endurance and sprint training

    VO2max during incremental exercise

  3. Body composition

    Time frame: Change from baseline at week 6 or 8 depending on the intervention

    Lean and fat mass during dual X-ray absorptiometry

  4. Myonuclei

    Time frame: Change from baseline at week 8 following resistance training

    Immunohistochemistry to determine number of myonuclei

  5. Satellite cells

    Time frame: Change from baseline at week 8 following resistance training

    Immunohistochemistry to determine number of satellite cells

  6. Fiber size

    Time frame: Change from baseline at week 8 following resistance training

    Immunohistochemistry to determine fiber size

Other outcomes

  1. Skeletal muscle mitochondrial supercomplexes

    Time frame: Change from baseline at week 6 following endurance and sprint training

    Pathway enrichment analysis of mitochondrial supercomplexes

Sponsors and collaborators

Lead sponsor

Morten Hostrup, PhD

Other

Registry information

Official study title

Proteome Remodelling and Dynamics in Human Skeletal Muscle: Effect of Training

Important dates

Study start
2019
Primary completion
2023
Study completion
2023
First posted
Oct 23, 2019
Registry last updated
Dec 27, 2023

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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