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Completed

NCT Number: NCT04279600

Taurine Supplementation and Training Effects on Energy Metabolism, Inflammation and Oxidative Stress in Obese Women

Taurine supplementation researches have increased due to its antioxidant and anti-inflammatory actions, and its ability to modulate lipid metabolism by stimulating the expression of proteins that regulates mitochondrial biogenesis and increases respiratory function (PGC-1α and PPAR) and irisin release when associated to exercise. Since obesity can induce metabolic disorders including abnormal production of adipokines and activation of pro-inflammatory signaling pathways also mitochondrial metabolism dysfunction in the adipose tissue, the use of taurine would be a new strategy for obesity prevention and treatment. Moreover, the association of taurine and exercise could improve exercise effects, promote higher energy expenditure and increase mitochondrial respiration, consequently resulting in weight loss. Therefore, the present investigation aims to evaluate the effects of the association of taurine supplementation and a combined exercise training protocol (aerobic and strength) on resting energy expenditure, weight, body composition, blood markers of inflammation and oxidative stress, telomeres length, and mitochondrial function and the expression of genes that regulates energy metabolism and lipid oxidation in the white adipose tissue in obese women.

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Key information

Conditions

Obesity Body Weight Nutrition Disorders Nutritional and Metabolic Diseases Overnutrition Overweight Pathological Conditions, Signs and Symptoms Signs and Symptoms

Age range

20 year–45 year

Sex eligibility

Female

Study type

Interventional

Phase

Not applicable

Primary location

School of Physical Education and Sport of Ribeirão Preto

Ribeirão Preto, São Paulo, 14040-907, Brazil

About this study

A double-blind placebo-controlled study was conducted with 24 obese women (32.9±6.3 years). Capsules of taurine (3 grams) (GTau) or placebo (GP) were daily supplemented 2 hours before training. The training program was composed of aerobic and strength exercises during one hour, 3 times a week, for an 8-week period (intensity of 80% heart rate). The taurine supplemented group received only taurine capsules (3g/day) during 8 weeks. Measurement of weight, hip and waist circumference, and body composition (by Deuterium oxide) were performed before and after the intervention. Resting energy expenditure and nutrients oxidation were assessed by calorimetry.

In order to check the effects of the intervention, abdominal tissue biopsy will be performed for white adipose tissue analysis, evaluation of mitochondrial function and quantification of the expression of genes related to energy metabolism and lipid oxidation and taurine pathway; blood collection will be done for quantification of taurine levels, inflammatory (IL-10, IL-15, IL-6, IL-1, TNF-α, and CRP), adipokines (adiponectin, adipsin, resistin, fetuin and leptin) and oxidative stress (GPx, SOD and MDA) markers. Also, evaluation of telomere length was performed. Body composition was evaluated by deuterium oxide method, weight, waist and hip circumference were accessed. All the measurements were performed before and after the intervention period.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Body Mass Index of 30 to 40 kg / m²
  • Sedentary
  • No associated co morbidity

Exclusion criteria

  • Women who have a medical impediment to the practice of physical exercise
  • Women that have undergone bariatric surgery
  • Menopause, cancer or any metabolic disease
  • Smokers
  • Alcoholics
  • Insulin-dependent diabetes

Treatment and study plan

Taurine

Dietary Supplement

Taurine supplementation in capsules of 1 gram of taurine powder, total dosage: 3 grams/day

Placebo

Dietary Supplement

Placebo supplementation in capsules of 1 gram of starch powder, total dosage: 3 grams/day

Exercise training

Other

4 weeks of combined exercise training (alternating strength and aerobic exercise), with a frequency of 3 times/week with 55 min/day.

Primary outcomes

  1. Change from baseline in white adipose tissue mitochondrial respiration at 8 weeks

    Time frame: eight weeks

    A subcutaneous adipose tissue sample collected for analysis of mitochondrial respiration (mitochondrial uncoupled state, phosphorylation state and electron transport system maximal capacity) were calculated at 8 weeks in comparison to the baseline.

  2. Change from baseline in indirect calorimetry at 8 weeks

    Time frame: eight weeks

    Change of energy expenditure and lipids oxidation were calculated at 8 weeks in comparision to the baseline.

  3. Changes from baseline in interleukines levels at 8 weeks

    Time frame: eight weeks

    Change of inflammatory markers such as interleukines 6, 10 and 15 were calculated at 8 weeks in comparision to the baseline.

  4. Changes from baseline in cytokine levels at 8 weeks

    Time frame: eight weeks

    Change of inflammatory markers such as adiponectin, resistin and adipsin were calculated at 8 weeks in comparision to the baseline.

  5. Changes from baseline in glutathione peroxidase levels at 8 weeks

    Time frame: eight weeks

    Change of oxidative stress markers such as glutathione peroxidase were calculated at 8 weeks in comparision to the baseline.

  6. Changes from baseline in superoxide dismutase levels at 8 weeks

    Time frame: eight weeks

    Change of oxidative stress markers such as superoxide dismutase were calculated at 8 weeks in comparision to the baseline.

  7. Changes from baseline in macronutrient intake at 8 weeks

    Time frame: eight weeks

    Change of macronutrient intake were calculated at 8 weeks in comparision to the baseline.

  8. Changes from baseline in total calorie intake at 8 weeks

    Time frame: eight weeks

    Change of total calorie intake were calculated at 8 weeks in comparision to the baseline.

  9. Changes from baseline in body composition at 8 weeks

    Time frame: eight weeks

    Change of body composition through deuterium oxide method were calculated at 8 weeks in comparision to the baseline.

Sponsors and collaborators

Lead sponsor

University of Sao Paulo

Other

Collaborators

  • Fundação de Amparo à Pesquisa do Estado de São Paulo

Registry information

Official study title

Taurine Supplementation and Physical Training Effects on Adipose Tissue Mitochondrial Energy Metabolism, and Blood Inflammation and Oxidative Stress in Obese Women

Acronym: Taurine

Important dates

Study start
2017
Primary completion
2017
Study completion
2018
First posted
Feb 21, 2020
Registry last updated
Feb 26, 2020

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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