Study of Two Different Putty Bone Grafts in Treatment of Intrabony Defects with the Aid of Intramarrow Penetration Technique

Inclusion criteria

Both genders with 25-55 years of age. Patients with intrabony defects in the Stage III Grade B periodontitis. Individuals with probing depth ≥ 5mm. Individuals with clinical attachment loss ≥ 5mm. Presence of at least one or more intrabony defect ≥ 3 mm on the radiograpgh patients who had not periodontal therapy in the previous 6 months.

Exclusion criteria

Patient with stage I, II and IV periodontitis. Pregnant or lactating mothers. Patients having any systemic disease. allergies to Local anesthesia and chlorhexidine, antibiotics, and analgesics will be excluded.

Clinical assessment:

including the following parameters: Plaque Index according to Silness P. Loe H 1964. 2. Gingival Index according Loe Silness 1963. 3. Probing pocket depth (PPD) is measured from the free gingival margin to the base of the pocket. 4. Clinical attachment level (CAL) is measured from the cement enamel junction to the base of the pocket.

• In patients All clinical assessments will be recorded at the baseline (immediate before surgery) and after 3 ,6 and 12months post-surgery and radiographic assessments will be recorded at the baseline and after 6 and 12months post-surgery.

This study was conducted following guidelines regulating research work on human subjects of the Faculty of Dentistry, Mansoura University, and the study protocol was reviewed and approved by the Research Ethics Committee of the Faculty of Dentistry, Mansoura University, Egypt. (Number:M0104023OM) All study subjects will sign written informal consent form before enrollment.

Materials:

• nanocrystalline hydroxyapatite in putty form (NanoBone® SBX Putty) is a unique and patented advanced bone grafting material. It incorporates nano-crystalline particles of hydroxyapatite (HA) that are similar in size, chemistry, and morphology to HA particles occurring in human bone. It is Synthetic biodegradable bone graft substitute consisting of non-sintered nanocrystalline hydroxyapatite in a porous silica gel matrix embedded in an aqueous gel
• Demineralized Bone Matrix allograft (DBM Putty). presurgical phase:

• Patients informed consents will be obtained prior to any periodontal treatment.
• Initial therapy includes full mouth supra and subgingival debridement using ultrasonic scalers and curettes
• and oral hygiene instruction will be given to all patients in the form of tooth brushing instructions and the use of chlorhexidine mouth wash 2 time daily for 2 weeks.
• After 4 weeks of initial therapy, all patients will be evaluated to ensure the behavior fitness for periodontal surgery.

surgical phase:

• Prior to surgery all patients will be given pre surgical rinse of 0.2 % Chlorhexidine for 30 seconds.
• The operative site will be anaesthetized using local anesthesia, Buccal and lingual crevicular incisions will be made.
• mucoperiosteal flaps will be reflected, the defect will be thoroughly debridement and root planning will be performed.
• The surgical area will be carefully irrigated with saline and root biomodification will be done.
• For both groups, decortication will be performed by penetrating the cortical walls of the intrabony defect by using a round carbide bur of 0.2 mm diameter to reach the marrow space.
• Multiple perforations will performed not closer than 1mm from each other and deep enough to obtain bleeding from the spongiosa
• The intrabony defects in Group I will be received nanocrystalline hydroxyapatite in putty form as grafting material (NanoBone® SBX Putty).
• Group II will be treated similarly as Group I and the same surgical protocol will followed except that the intrabony defects will be received Demineralized Bone Matrix allograft putty as grafting material, in both groups will be use membrane to cover of the graft materials and finally will be sutured the flap.

post-surgical phase:

• Non-steroidal anti-inflammatory drug and antibiotics will be prescribed.
• chlorhexidine digluconate rinses (0.12%) twice daily for 2 weeks.
• Patients will be advised to consume only soft food during the first week.
• After this period, patients will be reinstructed in mechanical cleaning of the treated teeth using a soft toothbrush and roll technique of brushing for 1 month.
• Sutures will be removed 10 days post operatively. All the patients will be recalled at 3, 6 and 12 months for periodic rechecking.

Statistical Analysis:

All data will be calculated and analyzed by suitable statistical tests

Inclusion criteria

• Both genders with 25-55 years of age.
• Patients with intrabony defects in the Stage III Grade B periodontitis.
• Individuals with probing depth ≥ 5mm.
• Individuals with clinical attachment loss ≥ 5mm.
• Presence of at least one or more intrabony defect ≥ 3 mm on the radiographs.
• patients who had not periodontal therapy in the previous 6 months.

Exclusion criteria

• Patient with stage I, II and IV periodontitis.
• Pregnant or lactating mothers.
• Patients having any systemic disease.
• allergies to Local anesthesia and chlorhexidine, antibiotics, and analgesics will be excluded.