Stem Cell Transplantation for Patients With Hematologic Malignancies

Secondary outcome evaluations for this clinical study include the following:

• To estimate the overall survival in patients with high risk hematological malignancies who receive a HSCT with an unmanipulated marrow graft or a peripheral blood stem cell graft
• To estimate disease-free survival and relapse rates
• To estimate the rates of chronic GvHD and graft failure
• To estimate the incidence of non-hematologic peri-transplant regimen-related toxicity and regimen-related mortality in the first 100 days after transplantation
• To estimate the time to neutrophil and platelet engraftment after transplantation
• To determine the degree of NK cell and T-cell immune reconstitution at 30 days and 100 days post-transplant
• To estimate the incidence of EBV reactivation or post-transplant lymphoproliferative disease (PTLPD)
• To determine the pharmacokinetics of anti-thymocyte globulin (rATG) in patients receiving allogeneic transplantation and the development of rATG antibodies

Originally this study began as a randomized comparison between unmanipulated bone marrow and T-cell depleted bone marrow utilizing the investigational CliniMACS selection system. The hypothesis to be tested at the time was that the incidence of severe acute GvHD was significantly reduced in children who received HSCT with a T-cell depleted bone marrow stem cell graft as compared to those receiving an unmanipulated graft. Approximately midway through the study the evidence indicated that although the incidence of severe acute GvHD with T-cell depletion was low, it was not significantly lower than the standard treatment of unmanipulated bone marrow. Therefore the study was amended to remove the T-cell depleted arm and continue accrual to one arm providing all patients with an unmanipulated bone marrow stem cell graft. The primary objective then being to determine if the true incidence of severe acute GvHD was below 15% as reported. The observational group receiving PBPC remained open for those patients whose donors or donor centers chose to provide PBPC in lieu of bone marrow. Only one such patient was assigned to this group; therefore, no valid conclusions can be formulated.

Intervention analysis was based on those patients who received an unmanipulated stem cell product only. For this study, the investigators had requested bone marrow for all study subjects. However, the final determination of the source of the hematopoietic stem cells, bone marrow or peripheral blood, was at the discretion of the donor and the donor center. Those participants who received a peripheral blood stem cell product were followed in the observational group only. All participants, whether recipients of a bone marrow or blood stem cell product, received the same preparative conditioning regimen

Inclusion criteria

• All patients lacking an HLA identical sibling for whom an unrelated donor matched at 6/6 HLA loci is formally requested within about 3 months of search initiation
• Age greater than or equal to 24 months, but less than 21 years for new patients.
• Diagnosis of one of the following high risk hematological malignancies:
• Acute lymphoblastic leukemia (in second or subsequent remission or high risk in first remission)
• Acute myeloid leukemia (in relapse or remission)
• Secondary AML/MDS
• Chronic myeloid leukemia
• Juvenile myelomonocytic leukemia
• Myelodysplastic syndrome
• Paroxysmal nocturnal hemoglobinuria
• Non-Hodgkin's lymphoma (in second or subsequent remission)

Exclusion criteria

• Patients with symptomatic cardiac disease, or evidence of significant cardiac disease by echocardiogram, or cardiac shortening fraction below 25%
• Patients with renal creatinine clearance < 40ml/min/1.73m^2
• Patients with FVC < 40% predicted or pulse oximetry less than or equal to 92% on room air if unable to perform pulmonary function tests
• Patients with direct bilirubin > 3 mg/dl
• Patients with SGPT > 500 U/L
• Patients with a Karnofsky or Lansky performance score < 70
• Patients who have received a previous allogeneic stem cell transplant
• Patients with a known allergy to rabbit or murine products
• Patients with isolated extramedullary leukemic relapse, including isolated CNS or testicular recurrence