Spirulina Platensis Supplementation and Calorie Restriction in Obese Men : A Randomized Controlled Trial Protocol Study

Spirulina supplement intervention on subjects with male gender aged 25-55 years, which will be intervened for 4 weeks / 30 days, before the intervention will be taken anthropometric parameters, body composition, lipid profile, insulin resistance, serum Superoxide Dismutase levels, and serum Malondialdehyde levels.

After that, spirulina supplement intervention will be given at a dose of 2x2 / day, and follow-up will be carried out every week, after 30 hours of intervention,spirulina supplement distribution is carried out by research assistants every 2 days.

Will be taken again to conduct anthropometric parameters, body composition, lipid profile, insulin resistance, serum Superoxide Dismutase levels, and serum Malondialdehyde levels.

The aim is to find out if there is an effect of spirulina supplementation on anthropometric parameters, body composition, lipid profile, insulin resistance, serum Superoxide Dismutase levels, and serum Malondialdehyde levels.

Inclusion criteria

• Male Age 25 - 55 years
• BMI ≥ 30 kg/m2
• Waist Circumference > 90 cm
• Low physical activity (Sedentary life style)
• If taking weight loss supplements are willing to stop the dietary supplements, food or drinks.
• Not undergoing a diet program.

Exclusion criteria

• History of kidney disease, atherosclerosis, cancer, and acute infections.
• Post-surgery in the last 3 months.
• Deficit in daily calorie requirement.
• Smoking habit and alcohol dependence.
• Use of hormonal drugs, antidepressant drugs, antibiotics, antidiuretic, glucocorticoids and use of vitamin or mineral supplements in the last three months.
• Refusal to sign the informed consent and in the study if not completing all stages will be excluded.