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OpenTrials
Completed

NCT Number: NCT02628249

Rate-limiting Amino Acids in Endurance-trained Athlete

Protein requirements in individuals who participate in endurance-based exercise training have been suggested to be greater than the current recommended dietary allowance (RDA).

The biological value of protein depends on the amino acid composition. As liebig's law of the minimum, the protein synthesis is limited due to the lack of single rate limiting amino acid. In this case, the first rate limiting amino acid determines protein intakes to maximize the protein synthesis. Adding a small amount of rate-limiting amino acid to the diet improves the biological value of its protein diet.

The indicator amino acid oxidation (IAAO) method has clarified the individual amino acid requirement in children, normal healthy adult and clinical populations. however, the IAAO method has never been utilized for determining the first rate limiting amino acid.

Therefore, the purpose of this study is to address of IAAO method to be applied for determining the rate-limiting amino acid in endurance athlete.

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Key information

Conditions

Increased Metabolic Requirement Healthy

Age range

18 year–35 year

Sex eligibility

Male

Study type

Interventional

Phase

Not applicable

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Gender: Male

Age limit: Minimum age 18 years - Maximum age 35 years

Accepts Healthy Volunteers : yes

Inclusion criteria

  • Endurance-trained participants who regularly more than 40 km/week
  • Ability to perform the exercise stimulus (20 km run) on metabolic trial.

Exclusion criteria

  • Inability to meet health and physical activity guidelines according to the physical activity readiness questionnaire (PAR-Q+)
  • Inability to adhere to any of the protocol guidelines (i.e. alcohol, caffeine consumption)
  • Regular tobacco use
  • Illicit drug use

Treatment and study plan

Base protein

Dietary Supplement

0.8 g/kg/d of protein provided as crystalline amino acid modeled after egg protein

Sufficient protein

Dietary Supplement

1.75 g/kg/d protein provided as crystalline amino acid modeled after egg protein.

Branched chain amino acids

Dietary Supplement

Branched chain amino acids modeled after egg protein.

Essential Amino Acids

Dietary Supplement

Essential amino acids modeled after egg protein.

Non essential amino acids

Dietary Supplement

Non essential amino acids modeled after egg protein.

Primary outcomes

  1. 13CO2 (carbon dioxide) excretion rate (μmol/kg/h)

    Time frame: At 8 hours after the end of exercise

    8 hours after exercise followed by ingesting 13C-labelled phenylalanine and one of 5 different amount of amino acids intake, 13CO2 excretion rate is determined by multiplying the enrichment of 13CO2 in breath measured by Mass spectrometry and CO2 production rate measured by metabolic cart.

Secondary outcomes

  1. [13C]phenylalanine oxidation rate (μmol/kg/h)

    Time frame: at 8 hours after the end of exercise

    8 hours after exercise followed by ingesting 13C-labelled phenylalanine and one of 5 different amount of amino acids intake, 13CO2 excretion rate is determined dividing 13CO2 excretion rate by urinary enrichment of 13C-phenylalanine in breath measured by Mass spectrometry, and CO2 production rate measured by metabolic cart.

Sponsors and collaborators

Lead sponsor

University of Toronto

Other

Registry information

Official study title

Application of Indicator Amino Acid Oxidation Method to Investigate the Rate-limiting Amino Acids in Endurance Trained Athlete

Acronym: EA

Important dates

Study start
2015
Primary completion
2017
Study completion
2017
First posted
Dec 11, 2015
Registry last updated
Apr 1, 2019

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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