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Completed

NCT Number: NCT05544955

Protein Ingestion Timing on Body Composition and Biochemical Markers in Resistance-trained Males

Protein ingestion timing is an important component for muscle accretion. The investigators hypothesized that protein ingestion timing could affect muscular adaptations and performance in resistance-trained men.

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Key information

Conditions

Hypertrophy Pathological Conditions, Anatomical Pathological Conditions, Signs and Symptoms Physical Performance

Age range

18 year–35 year

Sex eligibility

Male

Study type

Interventional

Phase

Not applicable

Primary location

Marymount University

Arlington, Virginia, 22207, United States

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • performing resistance training at least three times a week for 1 year prior to the start of the study
  • not taking any steroids or any illegal agents known to increase muscle size for at least 1 year prior to the start of the study
  • no known medical issues
  • sleeping for at least seven to eight hours during the 24-hour day

Exclusion criteria

  • non-willingness to continue protein ingestion or exercise protocols
  • participation in exercise other than the prescribed resistance training program
  • consumption of dietary supplement (other than the protein intervention) during the study period
  • Missing more than one resistance training session or protein ingestion throughout the study period

Treatment and study plan

isolate whey protein prior to and immediately after resistance training

Dietary Supplement

This arm involved protein ingestion immediately prior to and immediately after resistance training for 12 weeks. Participants will consume 40 g of isolate whey protein at their recommended timing.

isolate whey protein 3 hours prior to and 3 hours after resistance training

Dietary Supplement

This arm involved protein ingestion 3 hours prior to and 3 hours after resistance training for 12 weeks. Participants will consume 40 g of isolate whey protein at their recommended timing.

Primary outcomes

  1. Body Composition

    Time frame: 12 weeks

    Total Lean mass, fat mass, and estimated VAT (g) will be assessed using whole-body DXA

Secondary outcomes

  1. Muscular Strength

    Time frame: 12 weeks

    Using 1 repetition maximum

  2. Muscular Endurance

    Time frame: 12 weeks

    Using leg- and chest press exercises at 75% of the 1-RM

  3. Jump testing

    Time frame: 12 weeks

    Using the vertical and broad jump tests

  4. Pull-ups

    Time frame: 12 weeks

    Using the pull-up test

  5. Liver enzymes (ALT, AST, and GGT)

    Time frame: 12 weeks

    Using Fasting blood samples

  6. Creatinine and Urea Nitrogen

    Time frame: 12 weeks

    Urine samples will be collected using standard procedures

  7. Cholesterol Levels

    Time frame: 12 weeks

    Using Fasting blood samples

Sponsors and collaborators

Lead sponsor

Marymount University

Other

Collaborators

  • Shahid Beheshti University of Medical Sciences

Registry information

Official study title

Comparison of Protein Ingestion Timing on Body Composition, Physical Performance, and Biochemical Markers in Resistance-trained Males

Important dates

Study start
2022
Primary completion
2022
Study completion
2022
First posted
Sep 19, 2022
Registry last updated
Jun 11, 2025

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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