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NCT Number: NCT05835557

Physiological Outcomes of High-intensity Interval Training With Post-exercise Blood Flow Restriction

This research study revolves around the physiological outcomes of using post-exercise blood flow restriction (BFR) with high intensity short interval training (ST) The main aims of this study are to compare (i) aerobic performance outcomes, and (ii) anaerobic and muscular performance outcomes - between 2 groups of participants after either undergoing 3 weeks of ST + post-exercise BFR (BFR-ST) or traditional ST.

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Key information

Age range

18 year–40 year

Sex eligibility

Male

Study type

Interventional

Phase

Not applicable

Primary location

Human Bioenergetics Laboratory, National Institute of Education, Nanyang Technological University

Singapore, 637616

About this study

This research study revolves around the physiological outcomes of using post-exercise blood flow restriction (BFR) with high-intensity interval exercise training (HIIT), specifically high intensity short interval training (ST) protocol of 30s work, 30s rest, for the recreational endurance athlete. In previous research, it has been shown that using BFR during rest intervals of a ST protocol would elicit greater physiological stresses in terms of higher heart rate, greater muscle activation, reduced oxygenation within the muscle tissue during exercise, and increased blood lactate post-exercise as compared with the control condition. However, the physiological outcomes of this type of training method over a longer-term (9 sessions over 3 weeks) have not been explored. The main aims of this study is to compare the physiological outcomes, - (i) aerobic performance outcomes, and (ii) anaerobic and muscular performance outcomes - between 2 groups of participants after either undergoing 3 weeks of ST + post-exercise BFR (BFR-ST) or traditional ST . The hypotheses for this study are: the BFR-ST group would experience a (i) greater improvement in maximal aerobic capacity, function and time trial performance and, (ii) greater improvement in anaerobic power and capacity as well as muscular strength and power, as compared with the ST group.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Male
  • 18-40 years
  • Actively performing endurance exercise (running or cycling) for at least 2 sessions (1 - 1.5h) per week
  • Healthy (free from illnesses) and no musculoskeletal injuries for the past 6 months
  • No history of cardiometabolic, vascular diseases or similar conditions (e.g., high blood pressure, peripheral vascular diseases, heart diseases, metabolic syndrome, diabetes, stroke, etc.).
  • Pass the pre-participation health screening tests - Physical Activity Readiness Questionnaire for Everyone (PAR-Q+) and Australian Institute of Sport Blood Flow Restriction (AIS BFR) pre-screening questionnaire (no history of any risk factors).
  • Achieve a maximum oxygen uptake (VO2max) of ≥40ml/kg/min during a maximal aerobic power cycling screening test.
  • Non-smoker
  • Drink alcohol less than 3 times each week and less than 3 drinks each time

Exclusion criteria

  • Persons with any form of illnesses and/or musculoskeletal injuries for the past 6 months
  • Any history of cardiometabolic, vascular diseases or similar conditions which may worsen with the use of blood flow restriction during exercise
  • Fail the pre-participation health screening tests
  • Below 40ml/kg/min VO2max during maximal aerobic power cycling test
  • Smoker
  • Consumption of alcohol beyond that stated in the inclusion criteria.
  • History of asthma or taking medication for asthma.

Treatment and study plan

BFR-ST

Other

Undergo 9 sessions of high intensity short interval training involving 3 sets of 8-10 repetitions of 30 secs interval cycling and 30 secs recovery at 100-110% of maximal aerobic power. In between sets there will be 3 mins of passive rest. Blood flow restriction will be applied for 2 mins during the passive rest at 80% of limb occlusion pressure.

ST

Other

Undergo 9 sessions of high intensity short interval training involving 3 sets of 8-10 repetitions of 30 secs interval cycling and 30 secs recovery at 100-110% of maximal aerobic power. In between sets there will be 3 mins of passive rest. Cycling will be conducted in an environmental chamber and participants informed that the altitude is set to 2500m - actual altitude will be sea level.

Primary outcomes

  1. Maximum oxygen uptake and power

    Time frame: Will be assessed at baseline (Day 0 - Pre) and the change in this outcome 24 hours after 9 sessions of training over 3 weeks (Post)

  2. 20 km Cycling time trial

    Time frame: Will be assessed at baseline (Day 0 - Pre) and the change in this outcome 24 hours after 9 sessions of training over 3 weeks (Post)

  3. Wingate test of anaerobic power

    Time frame: Will be assessed at baseline (Day 0 - Pre) and the change in this outcome 24 hours after 9 sessions of training over 3 weeks (Post)

    Peak and mean power and rate of fatigue

Secondary outcomes

  1. Exercise economy

    Time frame: Will be assessed at baseline (Day 0 - Pre) and the change in this outcome 24 hours after 9 sessions of training over 3 weeks (Post)

    Oxygen uptake at a fixed load of 50% of maximum aerobic power

  2. Isometric mid-thigh pull

    Time frame: Will be assessed at baseline (Day 0 - Pre) and the change in this outcome 24 hours after 9 sessions of training over 3 weeks (Post)

    Maximal strength assessment of lower body

  3. Countermovement jump height

    Time frame: Will be assessed at baseline (Day 0 - Pre) and the change in this outcome 24 hours after 9 sessions of training over 3 weeks (Post)

    Vertical jump height assessment

Sponsors and collaborators

Lead sponsor

Nanyang Technological University

Other

Registry information

Official study title

Physiological Outcomes of High-intensity Interval Training With Post-exercise Blood Flow

Important dates

Study start
2023
Primary completion
2024
Study completion
2024
First posted
Apr 28, 2023
Registry last updated
Apr 13, 2025

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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