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Completed

NCT Number: NCT07181408

Perceived Satiety and Consumer Sensory Acceptance of Functional Foods Enriched With Edible Insect and Plant Proteins

This randomized, single-blind, triple crossover study investigated the effects of baked snacks containing either cricket flour (Acheta domesticus), pea flour (both at 15%), or a wheat flour-based control. One hundred adult participants were recruited and consumed each of the three products across separate sessions. Data were collected on sociodemographic characteristics, hunger and satiety perceptions using visual analogue scales (VAS), gastrointestinal tolerability via a post-consumption questionnaire, hedonic liking assessed with a 9-point scale, and sensory and emotional responses using Check-All-That-Apply (CATA) questionnaires.

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Key information

Conditions

Age range

18 year–65 year

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

Primary location

University of Torino

Torino, Italy

About this study

The present experimental study aimed to evaluate the subjective responses of consumers following the consumption of three types of savory snacks differing in protein composition: a conventional control snack, one enriched with cricket flour (Acheta domesticus), and one enriched with pea flour. The trial was designed as a randomized, single-blind, triple crossover intervention study. A total of 100 healthy adult volunteers were recruited, aged between 18 and 65 years, with a body mass index (BMI) ranging from 18.5 to 29.9 kg/m². Each participant consumed a single 50-gram portion of each product, with a seven-day washout period between sessions. The order of product administration was randomized. All tastings were carried out in a fasting state, preferably in the morning, to standardize metabolic conditions. During each session, the following data were collected:

  • Sociodemographic characteristics, assessed at baseline via questionnaire;
  • Perceived satiety, measured using a Visual Analogue Scale (VAS) at 60, 120, 180, 240, and 300 minutes after the end of the meal;
  • Gastrointestinal tolerability, evaluated through a post-consumption questionnaire addressing symptoms such as bloating, nausea, and abdominal discomfort;
  • Hedonic evaluation, assessed using a 9-point hedonic scale to determine product acceptability;
  • Sensory and emotional responses, collected through Check-All-That-Apply (CATA) questionnaires.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Healthy volunteer subjects aged between 18 and 65 years;
  • Body mass index (BMI) between 18.5 and 29.9 kg/m²

Exclusion criteria

  • Diagnosis of diabetes mellitus;
  • Thyroid disorders;
  • Food allergies;
  • Gastrointestinal and/or systemic diseases (including renal, hepatic, cardiovascular, or any other organ-related conditions);
  • Regular use of medications or dietary supplements;
  • Pregnancy or lactation;
  • Following specific dietary regimens (vegetarian or vegan).

Treatment and study plan

Wheat flour-based control snack

Other

Consumption of a single 50-gram portion of a wheat flour-based control snack.

Snack enriched with 15% cricket flour

Other

Consumption of a single 50-gram portion of a snack enriched with 15% cricket flour (Acheta domesticus).

Snack enriched with 15% pea flour.

Other

Consumption of a single 50-gram portion of a snack enriched with 15% pea flour.

Primary outcomes

  1. Postprandial Subjective Satiety Perception

    Time frame: 60, 120, 180, 240, and 300 minutes post-consumption.

    Satiety perception will be assessed using the Visual Analogue Scale (VAS; 0-100 mm), where 0 = "not satiated at all" and 100 = "extremely satiated". Higher scores indicate greater satiety.

  2. Postprandial Hunger

    Time frame: 60, 120, 180, 240, and 300 minutes post-consumption.

    Hunger perception will be assessed using the Visual Analogue Scale (VAS; 0-100 mm), where 0 = "not hungry at all" and 100 = "extremely hungry". Higher scores indicate greater hunger.

  3. Postprandial Gastric Fullness

    Time frame: 60, 120, 180, 240, and 300 minutes post-consumption.

    Gastric fullness perception will be assessed using the Visual Analogue Scale (VAS; 0-100 mm), where 0 = "not full at all" and 100 = "extremely full". Higher scores indicate greater fullness.

  4. Prospective Food Consumption

    Time frame: 60, 120, 180, 240, and 300 minutes post-consumption.

    Prospective food consumption will be assessed using the Visual Analogue Scale (VAS; 0-100 mm), where 0 = "no desire to eat at all" and 100 = "extreme desire to eat". Higher scores indicate greater desire for food intake.

Secondary outcomes

  1. Gastrointestinal Symptoms Evaluation

    Time frame: Within 24 hours after snack intake.

    Self-reported evaluation of gastrointestinal symptoms potentially related to snack consumption, assessed using the Gastrointestinal Symptom Rating Scale (GSRS). The GSRS uses a 10-point Likert scale (1 = no symptoms, 10 = very severe symptoms). Higher scores indicate more severe gastrointestinal symptoms.

  2. Sensory Acceptability

    Time frame: Immediately after snack consumption.

    Evaluation of overall sensory acceptability of the snack products, including visual appearance, odor, taste, texture, general liking, and purchase intent. Assessments will be performed using a Visual Analogue Scale (VAS; 0-100 mm), where 0 mm represents "dislike extremely / not acceptable at all" and 100 mm represents "like extremely / highly acceptable". Higher scores indicate greater sensory acceptability.

  3. Emotional and Descriptive Food Experience

    Time frame: Immediately after snack consumption.

    Exploration of the emotional and sensory descriptive dimension of the eating experience using Check-All-That-Apply (CATA) questionnaires. Participants will select all terms that describe their experience of the snack. Since this is a categorical measure, no numerical scale is applied. Data will be analyzed as the frequency of selection of each descriptor.

Sponsors and collaborators

Lead sponsor

University of Turin, Italy

Other

Registry information

Official study title

Assessment of the Impact of Functional Foods Enriched With Cricket and Pea Proteins on Perceived Satiety and Consumer Sensory Acceptance

Acronym: NOODLE

Important dates

Study start
2024
Primary completion
2025
Study completion
2025
First posted
Sep 18, 2025
Registry last updated
Sep 29, 2025

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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