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Completed

NCT Number: NCT04470505

Optimizing Platelet Isolation (The Cell Study)

The research study is about blood cells (platelets) and their power houses (mitochondria). The investigators want to learn about their behavior in order to ensure that our laboratory procedures are generating optimal results.

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Key information

Conditions

Healthy

Age range

25 year–35 year

Sex eligibility

All sexes

Study type

Observational

Primary location

Arkansas Children's Nutrition Center

Little Rock, Arkansas, 72202, United States

About this study

The objectives of this study are to assess platelet activation following standard laboratory procedures used in the process of measuring platelet mitochondrial function, and to determine the optimal amount of digitonin needed to permeabilize platelet cell membrane.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Age 25-35
  • All races
  • All ethnicity
  • 18.5 - 30 BMI

Exclusion criteria

  • smoking
  • Aspirin intake
  • Recreational drug use
  • Alcohol intake more than 3 times per week
  • Self-reported possibility of current pregnancy
  • Known diagnosis of:
  • Pre-diabetes
  • Diabetes
  • High cholesterol
  • High triglycerides
  • Hypertension
  • Anemia
  • Autism spectrum disorder
  • Attention deficit hyperactivity disorder (ADHD)
  • Attention deficit disorder (ADD)
  • Severe asthma
  • Refusal to stop taking nutritional supplements
  • Any other pre-existing medical condition that requires daily medication as determined by the investigators to affect outcome of interest

Treatment and study plan

Primary outcomes

  1. Platelet activation from isolated platelets

    Time frame: day 1

    Whole blood will be collected following an overnight fast, and platelets isolated using standard procedures. Platelet activation markers (e.g., CD62P ) will be measured using flow cytometric analysis.

Secondary outcomes

  1. Platelet activation measured in whole blood

    Time frame: day 1

    Whole blood will be collected following an overnight fast. Platelet activation will be measured in whole blood by the expression CD62P

  2. Platelet reactivity measured in whole blood

    Time frame: day 1

    Whole blood will be collected following an overnight fast. Platelet reactivity will be measured in whole blood with adenosine diphosphate (ADP).

Sponsors and collaborators

Lead sponsor

Arkansas Children's Hospital Research Institute

Other

Registry information

Official study title

Optimizing Platelet Isolation for Measures of Human Platelet Mitochondrial Respiration (The Cell Study)

Important dates

Study start
2020
Primary completion
2021
Study completion
2021
First posted
Jul 14, 2020
Registry last updated
Oct 20, 2025

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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