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Completed

NCT Number: NCT02918981

Nutritional Strategies to Augment the Postprandial Muscle Protein Synthetic Response to the Ingestion of a Low Dose of Protein in Middle-aged Women

Using stable isotope methodology, investigators will determine the postabsorptive and postprandial muscle protein synthetic response in 60 female adults (Age 50-79, BMI: <30 kg/m2) immediately after an acute bout of resistance exercise. Participants will be divided in to 5 groups of twelve women (n=12). Each group will receive a different protein beverage or placebo to consume following the resistance exercise training. Group 1 will consume a beverage consisting of whey protein; group 2 will consume whey protein supplemented with additional leucine; group 3 will consume whey protein supplemented with additional leucine and hydrolyzed whey peptides; group 4 will consume whey protein supplemented with additional leucine and citrulline; group 5 will just consume water. During the testing, blood and muscle samples will be collected.

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Key information

Conditions

Aging

Age range

20 year–79 year

Sex eligibility

Female

Study type

Interventional

Phase

Not applicable

Primary location

Freer Hall

Urbana, Illinois, 61801, United States

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • BMI <30 kg/m2
  • Healthy, sedentary
  • Either 20-30 years old or 50-79 years old

Exclusion criteria

  • Tobacco use
  • Allergies to milk consumption
  • Unusually high protein consumption
  • Vegan diet
  • Allergies to antibiotics or xylocaine
  • Phenylketonuria (PKU)
  • Diagnosed GI tract diseases
  • Arthritic conditions
  • A history of neuromuscular problems
  • Heart disease
  • Metabolic disorders
  • -Liver, kidney, or urinary disease
  • Musculoskeletal problems
  • Autoimmune disease
  • Neurological disease
  • Previous participation in amino acid tracer studies
  • Predisposition to hypertrophic scarring or keloid formation
  • Individuals on any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription strength acne medications).
  • Individuals using thyroid medications or other medications affecting endocrine function
  • Pregnancy
  • Contraindications for exercise
  • BMI ≥30
  • supplements that influence protein metabolism (e.g omega 3 fish oils)

Treatment and study plan

Resistance exercise

Behavioral

Participants will perform unilateral leg extension exercise immediately prior to ingestion of the experimental beverage

14 g Whey protein

Dietary Supplement

6.6 g Whey protein + 1.25 g leucine

Dietary Supplement

4 g Whey protein + 1.25 g leucine + 2.6 g Whey peptides

Dietary Supplement

6.6 g Whey protein + 1.25 g leucine + 0.8 g Citrulline

Dietary Supplement

Water Control

Other

Primary outcomes

  1. The myofibrillar protein synthetic response to protein ingestion

    Time frame: Postabsorptive for 3 hours, Postprandial for 4 hours hours

    Myofibrillar protein synthesis rates will be assessed during the postabsorptive period for 3 hr and during the 4 hr after the ingestion of the experimental beverages. This will allow us to assess the change from the postabsorptive to the postprandial period

Secondary outcomes

  1. mTORC1 phosphorylation

    Time frame: Baseline and at 2 and 4 hours after protein ingestion

    Activation of anabolic signaling pathways will be assessed in the fasted state and at 2 and 4 hr after the ingestion of the experimental beverages.

Sponsors and collaborators

Lead sponsor

University of Illinois at Urbana-Champaign

Other

Registry information

Important dates

Study start
2016
Primary completion
2019
Study completion
2019
First posted
Sep 29, 2016
Registry last updated
Nov 3, 2020

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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