Neutrophil Extracellular Traps in Systemic Sclerosis

This study is designed to assess the role of neutrophil extracellular traps (NETs) in systemic sclerosis as well as to evaluate the correlation between NETs production and NETs composition and the different complications and phenotypes observed in SSC.

30 SSC patients, 30 SLE patients and 60 healthy subjects will be recruited. Blood samples will be collected to obtain plasma, serum and polynuclear neutrophils by negative selection.

• The main aim of the study is to evaluate the quantity of NETs induced by serum from SSc patients on neutrophils from either healthy or SSC patients in vitro. The quantity of NETs produced by different populations of neutrophils in contact with sera from SSC will be compared with those produced by the same different populations of neutrophils in contact with sera from SLE, and healthy subjects (two control populations).
• Other objectives:

• To assess the composition of the NETs produced by different populations of neutrophils exposed to serum from SSC, SLE and healthy subjects.
• To correlate the quantity and the composition of NETS with clinical phenotype in SSc
• To assess the role of serum cytokines in Nets production in SSC.

Inclusion criteria

for patients of arm 1:

• patients with systemic lupus erythematosus
• patients consenting to participate to the study
• patients enrolled in the national healthcare insurance program

for patients of arm 2:

• patients with systemic sclerosis
• patients consenting to participate to the study
• patients enrolled in the national healthcare insurance program

For patients of arm 3 (healthy volunteers)

• Patients without Chronic inflammatory systemic disease
• Patients without Current or past neoplasy,
• patients without chronic metabolic pathology
• patients without treatment by anti inflammatory or corticotherapy for the last 15 days,
• patients without infectious pathology or inflammatory acute for the last 15 days