Neutrophil Extracellular Traps in Different Forms of Systemic Sclerosis

This study is designed to assess the role of Neutrophil Extracellular Traps (NETs) in Systemic SClerosis (SSC) as well as to evaluate the correlation between NETs production and NETs composition and the different complications and phenotypes observed in SSC.

100 SSC patients, 30 other connective tissue disease patients and 130 healthy subjects will be recruited. Blood samples will be collected to obtain plasma, serum and polynuclear neutrophils by negative selection.

Inclusion criteria

*For patients of group 1:

• Patients with systemic sclerosis (ACR-EULAR Criteria)
• Patients taking care of in internal medicine or in dermatology department's of the university hospital of Reims
• Patients consenting to participate to the study
• Patients enrolled in the national healthcare insurance program

For patients of group 2:

• Patients with other connective tissue disease (ACR specific-disease criteria)
• Patients taking care of in internal medicine or in dermatology department's of the university hospital of Reims
• Patients consenting to participate to the study
• Patients enrolled in the national healthcare insurance program

For patients of group 3 (healthy volunteers)

• Patients eligible for blood donation (blood donation regulation criteria of January 11th 2022 decree)
• Patients without medical history of autoimmune systemic or chronic inflammatory systemic disease,
• Patients without current or past neoplasy disease,
• Patients without chronic metabolic pathology
• Patients without treatment by anti inflammatory or corticotherapy for the last 15 days,
• Patients without infectious pathology or inflammatory acute for the last 15 days
• Patients consenting to participate to the study

Exclusion criteria

for patients of all groups:

• Patients/Healthy volunteers under 18 years old
• Patients/Healthy volunteers protected by the law
• Patients/Healthy volunteers not consenting to participate to the study after information
• Patients with inflammatory pathology or infectious acute intercurrent pathology in the last 15 days before inclusion
• Pregnant or breastfeeding women