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Completed

NCT Number: NCT03879187

Molecular and Hormonal Responses to Diet-Induced Insulin Resistance

This study determined the effect of 7 days of high-fat overfeeding on whole-body glycaemic control, glucose kinetics, skeletal muscle insulin signalling, and markers of skeletal muscle microvascular function in 15 healthy young individuals.

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Key information

Conditions

Healthy Glucose Metabolism Disorders Hyperinsulinism Insulin Resistance Metabolic Diseases Nutritional and Metabolic Diseases

Age range

18 year–40 year

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

About this study

Fifteen healthy, young individuals underwent metabolic testing before and after 7 days of high-fat overfeeding. We determined whole-body glycaemic control, glucose kinetics, skeletal muscle insulin signalling, and markers of skeletal muscle microvascular function. Stable isotope tracers were used to assess the rate of appearance of glucose from the gut, liver glucose output and whole-body glucose disposal. Insulin signalling was determined by Western blotting of skeletal muscle tissue. Microvascular function was assessed through the use of quantitative immunofluorescence microscopy. These measurements were performed both postabsorptively and following the ingestion of 50 grams of glucose + 15 grams of protein.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Aged between 18 and 40 years
  • Physically active
  • Non-smoker
  • Not taking any medication known to interfere with the study
  • No history of cardiovascular disease or diabetes
  • Weight stable for the last 6 months

Exclusion criteria

  • Outside of age range
  • Sedentary
  • Smoker
  • Taking medication known to interfere with the study
  • History of cardiovascular disease or diabetes
  • Unstable weight within last 6 months

Treatment and study plan

High-fat, high-energy diet

Dietary Supplement

Participants consumed a diet that was high in fat and high in energy

Primary outcomes

  1. Change in glucose concentration

    Time frame: Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)

    Venous blood samples were obtained the day before and the day after the 7 day high-fat diet. Glucose was reported as mmol/L.

  2. Change in insulin concentration

    Time frame: Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)

    Venous blood samples were obtained the day before and the day after the 7 day high-fat diet. Insulin was reported as pmol/L.

  3. Change in glucose kinetics (i.e., rate of appearance and disappearance) assessed using isotope tracers

    Time frame: Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)

    Glucose kinetics were assessed the day before and the day after the 7 day high-fat diet. Data expressed as umol/kg/min.

  4. Change in skeletal muscle insulin signalling

    Time frame: Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)

    AKT and AS160 phosphorylation was determined by Western blotting of skeletal muscle. Data are expressed as arbitrary units.

  5. Change in markers of microvascular function

    Time frame: Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)

    endothelial nitric oxide synthase was determined in terminal arterioles by quantitative immunofluorescence microscopy that was performed on sections of skeletal muscle tissue. Data presented as arbitrary units.

Secondary outcomes

  1. Change intramuscular triglyceride content

    Time frame: Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)

    Intramuscular triglyceride content was determined by quantitative immunofluorescence microscopy that was performed on sections of skeletal muscle tissue. Data presented as arbitrary units.

Sponsors and collaborators

Lead sponsor

Loughborough University

Other

Registry information

Important dates

Study start
2015
Primary completion
2018
Study completion
2018
First posted
Mar 18, 2019
Registry last updated
Mar 18, 2019

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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