Investigation of Antibacterial Effect of Levobupivacaine on Staphylococcus Aureus

Study solutions were prepared as 100 mL each. Four groups were designed:

• Group 1: 0.125% levobupivacaine + 4 mL fentanyl (200 mcg)
• Group 2: 0.0625% levobupivacaine + 4 mL fentanyl (200 mcg)
• Group 3: 4 mL fentanyl (200 mcg)
• Group 4: 99 mL saline (control)

Each group received 1 mL of Staphylococcus aureus 0.5 McF (1.5×10⁸ cfu/mL). All samples were infused through a Portex bacterial filter at 4 mL/h for 24 hours using PCA devices. Samples collected from bottles, filter inlets, and outlets were cultivated on Blood Agar. Colony counts were compared statistically using the Kruskal-Wallis and Mann-Whitney U tests (p<0.05).

Inclusion criteria

• Laboratory study using Staphylococcus aureus bacterial cultures.
• Samples exposed to different concentrations of levobupivacaine and fentanyl solutions.
• Controlled laboratory environment (Dokuz Eylül University, Microbiology Laboratory).

Exclusion criteria

• No human participants included.
• No animal models used.
• Studies outside the defined experimental conditions were excluded.