Skip to main content
OpenTrials
Completed

NCT Number: NCT02812446

Staphylokinase and ABO Group Phenotype: New Players in Staphylococcus Aureus Implant-associated Infections Development

The purpose of this study is to identify bacterial and/or clinical features involved in the pathogenesis of Staphylococcus aureus implant-associated infections (IAI). Materials & methods: In total, 57 IAI S. aureus and 31 nasal carriage (NC) S. aureus isolates were studied. Staphylococcus aureus genetic background was obtained by microarray analysis. Multilocus sequence typing was performed to determine clonal complexes (CC). Biofilm production was investigated by resazurin and crystal violet methods

Completed

Looking for future studies?

Notify Me

Key information

Conditions

Staphylococcal Infection Bacterial Infections Bacterial Infections and Mycoses Gram-Positive Bacterial Infections Infections Staphylococcal Infections

Sex eligibility

All sexes

Study type

Observational

Primary location

CHU de Nantes

Nantes, 44093, France

Who can participate

Healthy volunteers accepted: No

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • infected patients with S. Aureus IAI infection
  • noninfected patients screened for S. aureus nasal colonization at the orthopedic consultation ward

Exclusion criteria

  • minor

Treatment and study plan

IAI infection

Other

Staphylococcus aureus nasal carriage infection

Other

Primary outcomes

  1. biofilm production measurement in the two groups

    Time frame: september 2012

    The primary aim of this study is to identify phenotypic (biofilm production) markers in two different groups of S. aureus strains isolated from patients with IAI or screened from nasal carrier in noninfected patients undergoing orthopedic surgery.

    By using resazurin method, IAI and NC nasal carriage isolates were divided into three groups. Isolates were classified as strong, moderate and weak biofilm producers.

  2. Clonal complexes distribution

    Time frame: september 2012

    The primary aim of this study is to identify genotypic markers in two different groups of S. aureus strains isolated from patients with IAI or screened from nasal carrier in noninfected patients undergoing orthopedic surgery.

    IAI and NC isolates genetic background was determined in order to compare the prevalence of individual virulence factor genes. All genes provided by the Alere StaphyType DNA microarray were studied.

  3. IAI and NC nasal carriage isolates genetic background

    Time frame: september 2012

    IAI and NC isolates genetic background was determined in order to compare the prevalence of individual virulence factor genes. All genes provided by the Alere StaphyType DNA microarray were studied

Secondary outcomes

  1. biofilm production correlation with genotype

    Time frame: december 2013

    quantile-quantile (QQ)-plot analysis

  2. Correlation between Clonal Complexes distribution , genotype and clinical parameters

    Time frame: december 2013

    In order to determine whether clusters of S. aureus could be associated with clinical characteristics of the patients, search of association between CCs and clinical data was performed

  3. Association of ABO group phenotype and IAI S. aureus genotype

    Time frame: december 2013

    QQ-plots analysis

Sponsors and collaborators

Lead sponsor

Nantes University Hospital

Other

Registry information

Important dates

Study start
2012
Primary completion
2012
Study completion
2013
First posted
Jun 24, 2016
Registry last updated
Jun 24, 2026

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

Related clinical trials

Published trials that share one or more normalized conditions with this study.