Interval Training and Cardio-metabolic Health in Overweight/Obese Girls

measures of weight in kilograms, height in meters, calculation of BMI in kg/m^2)

will be assessed using an Alinity-Abbott autoanalyzer

Enjoyment will be measured using the validated Physical Activity Enjoyment Scale. This scale was used to assess the level of enjoyment following interventions using the participants' responses to 18 items rated on a 7-point bipolar rating scale. The assessment consists of questions relateding to the enjoyment after intervention with the instruction, "Please rate how you feel about the physical activity you have been doing at the moment about the physical activity you have been doing". An overallOverall enjoyment of physical activity score was generated by summing the individual item scores. Scores ranged from 18-126, with higher scores reflecting higher levels of enjoyment.

Aerobic velocity will be measured with incremental running test "the Spartacus test". This test is validated for the evaluation of aerobic capacity for obese adolescents. A rectangle of 750 m (75 × 10 m) will be created with different marks set at regular intervals, which represent the different speeds (from 7 to 18 km/h). Each stage lasts three minutes whereby the first stage is set at 7 km/h and each following stage increasing by 1 km/h. During each stage, the participant had 15 s to reach the corresponding mark and then 15 s of rest. Participants adjusted their running speed simultaneously to cones and audio beep. The test ended when the subject was no longer able to maintain the pace for two consecutive occasions or when he decided to stop due to exhaustion.

Lipidomic profile will be assessed by measuring selected plasma polyunsaturated fatty acids (PUFAs), oxylipins and endocabnnabinnoids by LC-MS/MS method

Hematological parameters included red blood cell count, hemoglobin, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, leukocyte count , neutrophil count , lymphocyte count, monocyte count , eosinophil count and platelet count will be measured using an automated cell counter (Sysmex XN450; Norderstedt, Germany).

Heart rate will be recorded using a heart rate monitor (S810, Polar, Kempele, Finland) during the incremental running test "the Spartacus test".

Sprinting performance will be evaluated by 10-m, 20-m, 30 --m and 60-m sprints, using a series of paired photocells with an accuracy of 0.01 s (Brower Timing System, Salt Lake City, UT).