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NCT Number: NCT07503860

Indirect Biomarkers and Exercise Induced Muscle Damage

The purpose of this study is to investigate the magnitude of changes in biomarkers of exercise-induced muscle damage following an acute bout of eccentric exercise of the knee extensors performed on an isokinetic dynamometer. Moreover, these biomarkers will be assessed at different time points after the initial intervention, checking the repeated bout effect phenomenon.

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This study is active but is not currently recruiting participants.

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Key information

Conditions

Exercise Induced Muscle Damage

Age range

18 year–30 year

Sex eligibility

Male

Study type

Interventional

Phase

Not applicable

Primary location

Department of Physical Education and Sport Science, National and Kapodistrian University of Athens

Athens, Attica, 17237, Greece

About this study

Volunteers will visit the laboratory a total of two times. During the first visit, in all participants will be assessed indirect markers of exercise-induced muscle damage (i.e., peak torque, delayed-onset muscle soreness, range of motion, rate of force development), blood parameters (i.e., creatine kinase and c-Reactive Protein), and muscle oxygenation using near infrared spectroscopy. Afterwards, participants will perform an eccentric exercise protocol for the knee extensors consisting of 5 sets of 15 maximal-intensity repetitions at an angular velocity of 60°/s. The second visit will be at different time points between participant, where the indirect mauls damage biomarkers will be reassessed before and after the same isokinetic eccentric exercise (i.e., 5 sets of 15 maximal-intensity repetitions at an angular velocity of 60°/s).

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Healthy, physically active, males

Exclusion criteria

  • Musculoskeletal injuries, chronic diseases, smoking, consumption of any sports/antioxidant supplements or medication, and potential inflammatory conditions

Treatment and study plan

Eccentric exercise

Other

Eccentric exercise will be performed using an isokinetic dynamometer. It will be consisted of 5 set of 15 eccentric contraction of the knee extensors at an angular velocity of 60o/s. The intensity will be maximal voluntary.

Primary outcomes

  1. Delayed onset muscle soreness

    Time frame: Baseline and 48 hours after the eccentric exercise bout.

    Pain will be assessed at baseline (before the eccentric exercise bout) and again at 48 hours (2 days) after the eccentric exercise bout.

  2. Peak torque output

    Time frame: Baseline and 48 hours after the eccentric exercise bout.

    Peak torque will be assessed at baseline (before the eccentric exercise bout) and again at 48 hours (2 days) after the eccentric exercise bout.

  3. Range of Motion

    Time frame: Baseline and 48 hours after the eccentric exercise bout.

    The free pain range of motion will be assessed at baseline (before the eccentric exercise bout) and again at 48 hours (2 days) after the eccentric exercise bout.

  4. Muscle oxygenation

    Time frame: Baseline and 48 hours after the eccentric exercise bout.

    Using near infrared spectroscopy, the oxygenation of the knee extensors will be assessed at baseline (before the eccentric exercise bout) and again at 48 hours (2 days) after the eccentric exercise bout.

  5. Creatine kinase

    Time frame: Baseline and 48 hours after the eccentric exercise bout.

    Creatine kinase will be assessed at baseline (before the eccentric exercise bout) and again at 48 hours (2 days) after the eccentric exercise bout.

  6. Muscle thickness using ultrasound

    Time frame: Baseline and 48 hours after the eccentric exercise bout.

    Participants's knee extensors muscle thickness will be assessed at baseline (before the eccentric exercise bout) and again at 48 hours (2 days) after the eccentric exercise bout.

Sponsors and collaborators

Lead sponsor

Vassilis Paschalis

Other

Registry information

Official study title

Repeated Bout Effect and Indirect Biomarkers After Eccentric Exercise Induced Muscle Damage

Important dates

Study start
2025
Primary completion
2025
Study completion
2026
First posted
Mar 31, 2026
Registry last updated
Mar 31, 2026

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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