In Vitro Model of the Cystic Fibrosis Bronchial Epithelium Via iPS Technology

The investigator's primary objective is to generate iPS lines from 3 CF patients and from 3 healthy subjects.

Secondary objectives include verification that cell lines express the CFTR gene according to their genotype, verification or relative production of the CFTR protein for each iPS line, and amplification of obtain iPS lines for aliquot creation to facilitate sharing.

Inclusion criteria

for subjects with Cystic Fibrosis:

• Homozygote for the p.Phe508del mutation
• Signed informed consent given by the subject

Inclusion criteria

for subjects without Cystic Fibrosis:

• Signed informed consent given by the subject

Exclusion criteria

• Pregnancy, breastfeeding
• Participant in an exclusion period determined by a previous study
• Participant under any kind of guardianship
• Unaffiliated with or not a beneficiary of a social security program (health insurance)
• Subject deprived of liberty (e.g. prisoners)
• Subject with positive infectious markers for HIV1, HIV2, HBC or HBV

Exclusion criteria

for subjects with Cystic Fibrosis:

• Any pathology requiring a treatment or a pathology not requiring treatment but with clinical significance according to the investigator