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Completed

NCT Number: NCT04850820

Impact of Amino Acids to Enhance Anabolism After Home-based Exercise

An adequate quantity of lean body mass is vital for optimal health and performance, and is accrued when net protein balance (NPB) is positive. Amino acids are the building blocks of protein, and when consumed following resistance exercise, significantly improve NPB.

However, no study has investigated how a supplement of all of the essential amino acids (EAA) affects NPB following resistance exercise in a free-living, home-based setting, particularly when compared to a supplement of branched-chain amino acids (BCAA).

Therefore, the purpose of this study is to determine the impact of a novel EAA supplement on anabolism (e.g., NPB) as compared to BCAA and placebo supplements following home-based resistance exercise.

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Key information

Conditions

Amino Acids, Essential Behavior Exercise Metabolism Motor Activity

Age range

18 year–35 year

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

Primary location

Goldring Centre for High Performance Sport

Toronto, Ontario, M5S2C9, Canada

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Aged 18-35 years
  • Recreationally active; currently performing structured exercise (e.g., running, weight lifting, team-sport activity) at least 2 days per week

Exclusion criteria

  • Body Mass Index: >29.9kg/m^2
  • Inability to perform physical activity as determined by the Physical Activity Readiness Questionnaire
  • Inability to adhere to protocol guidelines (e.g., alcohol, habitual diet)
  • Regular tobacco use
  • Illicit drug use (e.g., growth hormone, testosterone)
  • Diagnosed medical condition under the care of a physician
  • Inability to abstain from supplements (e.g., protein, creatine, HMB, BCAA, phosphatidic acid) at least three weeks before and during trials
  • Individuals on any medications known to affect protein metabolism (e.g., corticosteroids, non-steroidal anti-inflammatories, or prescription-strength acne medications)

Treatment and study plan

Novel essential amino acid supplementation

Dietary Supplement

This intervention will determine the impact of EAA+ on markers of whole-body net protein balance, protein synthesis, and protein breakdown following home-based resistance exercise in a free-living setting.

Branched-chain amino acid supplementation (BCAA)

Dietary Supplement

This intervention will determine the impact of the BCAA supplement (to be compared to EAA+) on markers of whole-body net protein balance, protein synthesis, and protein breakdown following home-based resistance exercise in a free-living setting.

Isocaloric carbohydrate supplementation

Dietary Supplement

This intervention will determine the impact of an isocaloric carbohydrate placebo supplement (to be compared to EAA+) on markers of whole-body net protein balance, protein synthesis, and protein breakdown following home-based resistance exercise in a free-living setting.

Primary outcomes

  1. Whole-body net leucine balance (umol/kg)

    Time frame: 5 hours/trial (four trials total)

    Used as a proxy of whole-body protein balance, and estimated by using [13C]leucine ingestion and breath/urine sample collection

Secondary outcomes

  1. Urinary 3-methylhistidine (3-methylhistidine:creatinine ratio)

    Time frame: 5 hours/trial (four trials total)

    Used as a marker of muscle protein breakdown by measuring the 3-methylhistidine:creatinine ratio in the urine

Sponsors and collaborators

Lead sponsor

University of Toronto

Other

Collaborators

  • Iovate Health Sciences International Inc

Registry information

Acronym: B2B

Important dates

Study start
2021
Primary completion
2021
Study completion
2021
First posted
Apr 20, 2021
Registry last updated
May 5, 2022

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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