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Completed

NCT Number: NCT05707455

Hematological Factors and Iron Status in Aerobic Versus Anaerobic Training in Athletic Females

This study aimed to evaluate the hematological factors and iron status in aerobic versus anaerobic training in athletic females.

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Key information

Conditions

Sports Anemia

Age range

16 year–19 year

Sex eligibility

Female

Study type

Observational

Primary location

Local clubs

Cairo, Egypt

About this study

In both the short and long term, physical training causes iron status deterioration in sporty females. Nonetheless, little is known about the effect of different forms of exercise (aerobic versus anaerobic) on haematological variables and iron status in sporty females during adolescence. As a result, the purpose of this study was to compare the haematological variables and iron status in aerobic versus anaerobic exercise in adolescent female athletes. Another goal was to compare aerobic and anaerobic groups in terms of iron status classifications, such as normal iron status, iron deficiency with or without anaemia.

Who can participate

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Healthy, virginal, nonsmoker, female athletes.
  • Age from 16 to 19 years.
  • Body mass index < 25 kg/m2.
  • Having the same socio-economic level.
  • Following a normal balanced nutrition.

Exclusion criteria

  • Using any medical or hormonal therapy that might influence the iron status.
  • Vegetarian athletes.
  • Having menorrhagia or amenorrhea.
  • Chronic inflammatory condition or existing infection.
  • Diabetic, hypertensive or cardiac athletes.
  • Haematological disease (with the exception of iron deficiency with or without anaemia).
  • Having transfusion of blood.

Treatment and study plan

evaluating blood levels of hemoglobin, hematocrit, red blood cell count, mean corpuscular volume, mean corpuscular hemoglobin concentration, serum transferrin and serum ferritin.

Other

evaluating blood levels of hemoglobin, hematocrit, red blood cell count, mean corpuscular volume, mean corpuscular hemoglobin concentration, serum transferrin and serum ferritin.

Primary outcomes

  1. Assessment of hemoglobin (Hb)

    Time frame: 2 months

    Blood samples were collected between 8:00 and 9:00 a.m. after an overnight fast and time of relaxation. The athletic girls were asked to refrain from exercising the day before sample. Blood was taken from the antecubital vein and placed in one EDTA tube and one beads tube for serum separation. An automatic cell counter (Sysmex XS 1000, Japan) was used to measure hemoglobin (Hb).

  2. Assessment of hematocrit (Hct)

    Time frame: 2 months

    Blood samples were collected between 8:00 and 9:00 a.m. after an overnight fast and time of relaxation. The athletic girls were asked to refrain from exercising the day before sample. Blood was taken from the antecubital vein and placed in one EDTA tube and one beads tube for serum separation. An automatic cell counter (Sysmex XS 1000, Japan) was used to measure hematocrit (Hct).

  3. Assessment of red blood cell (RBC) count

    Time frame: 2 months

    Blood samples were collected between 8:00 and 9:00 a.m. after an overnight fast and time of relaxation. The athletic girls were asked to refrain from exercising the day before sample. Blood was taken from the antecubital vein and placed in one EDTA tube and one beads tube for serum separation. An automatic cell counter (Sysmex XS 1000, Japan) was used to measure red blood cell (RBC) count.

  4. Assessment of mean corpuscular volume (MCV)

    Time frame: 2 months

    Blood samples were collected between 8:00 and 9:00 a.m. after an overnight fast and time of relaxation. The athletic girls were asked to refrain from exercising the day before sample. Blood was taken from the antecubital vein and placed in one EDTA tube and one beads tube for serum separation. An automatic cell counter (Sysmex XS 1000, Japan) was used to measure mean corpuscular volume (MCV).

  5. Assessment of mean corpuscular hemoglobin concentration (MCHC)

    Time frame: 2 months

    Blood samples were collected between 8:00 and 9:00 a.m. after an overnight fast and time of relaxation. The athletic girls were asked to refrain from exercising the day before sample. Blood was taken from the antecubital vein and placed in one EDTA tube and one beads tube for serum separation. An automatic cell counter (Sysmex XS 1000, Japan) was used to measure mean corpuscular hemoglobin concentration (MCHC).

  6. Assessment of serum transferrin

    Time frame: 2 months

    Blood samples were collected between 8:00 and 9:00 a.m. after an overnight fast and time of relaxation. The athletic girls were asked to refrain from exercising the day before sample. Blood was taken from the antecubital vein and placed in one EDTA tube and one beads tube for serum separation. The serum sample was centrifuged, and the serum transferrin was measured using COBAS Integra 400 plus, Switzerland.

  7. Assessment of serum ferritin

    Time frame: 2 months

    Blood samples were collected between 8:00 and 9:00 a.m. after an overnight fast and time of relaxation. The athletic girls were asked to refrain from exercising the day before sample. Blood was taken from the antecubital vein and placed in one EDTA tube and one beads tube for serum separation. The serum sample was centrifuged, and the serum ferritin was measured using ADVIA Centaur XPT, Germany.

Secondary outcomes

  1. Anthropometric measures

    Time frame: 2 months

    A weight-height scale was used to assess the weight (Kg) and height (m) for each female athlete in the two groups. The body mass index (BMI) was then calculated by dividing the weight by the height squared (Kg/m2).

  2. Assessment of participation age to sport

    Time frame: 2 months

    Each female athlete was asked about her age of participation to sport to assess her training profile.

  3. Assessment of number of hours of training per week

    Time frame: 2 months

    Each female athlete was asked about the number of hours of training per week to assess her training profile.

  4. Assessment of training experience

    Time frame: 2 months

    The training experience in years was calculated by subtracting the female athlete's age of participation to training from her chronological age.

Sponsors and collaborators

Lead sponsor

Cairo University

Other

Registry information

Official study title

Hematological Factors and Iron Status in Aerobic Versus Anaerobic Training in Athletic Females: An Observational Study

Important dates

Study start
2022
Primary completion
2022
Study completion
2022
First posted
Feb 1, 2023
Registry last updated
Sep 26, 2023

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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