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Completed

NCT Number: NCT03717948

Examining the Impact of Blood Flow Restricted Aerobic Exercise on PGC-1α mRNA Expression in Young Healthy Males

This crossover study investigates the effects of restricting arterial blood flow during cycling exercise on PGC-1α and its upstream signalling pathways. In a randomized order, participants will exercise once for 30 minutes with restricted blood flow and then will exercise once for 30 minutes with no blood flow restriction.

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Key information

Conditions

Mitochondrial Biogenesis

Age range

18 year–30 year

Sex eligibility

Male

Study type

Interventional

Phase

Not applicable

Primary location

Queen's University

Kingston, Ontario, K7L3N6, Canada

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • 3 hours of physical activity per week
  • No previous cycling training
  • No concurrent involvement in another training program
  • Body mass index < 30 kg/m2

Exclusion criteria

  • Presence of cardiovascular disease
  • Presence of metabolic disease
  • Taking regular oral medication
  • Current smoker

Treatment and study plan

BFR Exercise

Other

Participants will exercise on a recumbent bike placed on a 45 degree incline (BFR).

CTL Exercise

Other

Participants will exercise on a recumbent bike placed on flat ground (CTL).

Primary outcomes

  1. Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) messenger ribonucleic acid (mRNA)

    Time frame: 15 minutes before exercise and 3 hours post-exercise for both exercise sessions

    Fold-change in PGC-1α mRNA expression

Secondary outcomes

  1. Catecholamine concentrations

    Time frame: 15 minutes before exercise and immediately post-exercise for both exercise sessions

    Catecholamine concentrations in venous blood

  2. Total and phosphorylated p38 mitogen-activated protein kinase (MAPK)

    Time frame: 15 minutes before exercise and immediately post-exercise for both exercise sessions

    Fold change in p38 MAPK phosphorylation

  3. Total and phosphorylated acetyl coenzyme A carboxylase (ACC)

    Time frame: 15 minutes before exercise and immediately post-exercise for both exercise sessions

    Fold change in ACC phosphorylation

  4. Total and phosphorylated protein kinase A (PKA)

    Time frame: 15 minutes before exercise and immediately post-exercise for both exercise sessions

    Fold change in PKA phosphorylation

  5. Acetylated p53

    Time frame: 15 minutes before exercise and immediately post-exercise for both exercise sessions

    Fold change in acetylated p53

  6. Blood lactate

    Time frame: During both exercise sessions (lactate); 15 minutes before and immediately after both exercise sessions

    Average [lactate] between conditions

  7. Heart rate (HR)

    Time frame: During both exercise sessions

    Average HR between conditions

  8. Revolutions per minute (RPM)

    Time frame: During both exercise sessions

    Average RPM between conditions

  9. Ratings of perceived exertion (RPE)

    Time frame: During both exercise sessions

    Average RPE between conditions

  10. Oxygenated and deoxygenated hemoglobin

    Time frame: During both exercise sessions and up to 10 minutes after (for cuff occlusion)

    Average oxygenated and deoxygenated hemoglobin as a percentage of same day max values

Sponsors and collaborators

Lead sponsor

Queen's University

Other

Registry information

Official study title

Examining the Impact of Acute Blood Flow Restricted Aerobic Exercise on PGC-1α mRNA Expression in Young Healthy Males: A Randomized Cross-over Trial

Important dates

Study start
2018
Primary completion
2019
Study completion
2019
First posted
Oct 24, 2018
Registry last updated
Jul 15, 2019

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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