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NCT Number: NCT04151095

Examining The Effect of Blood Flow Restricted Aerobic Training on Mitochondrial Adaptations in Young Healthy Adults

This parallel-arm trial study investigates the effects of restricting arterial blood flow during cycling exercise on citrate synthase. Participants will be randomized to either a blood flow restricted or non-blood flow restricted exercise training group. Both groups will complete seven, 30-minute exercise sessions over a 14-day period.

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Key information

Conditions

Mitochondrial Biogenesis

Age range

18 year–30 year

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

Primary location

Queen's University

Kingston, Ontario, K7L3N6, Canada

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • 3 hours of physical activity per week
  • No previous cycling training
  • No concurrent involvement in another training program
  • Body mass index < 30 kg/m2

Exclusion criteria

  • Presence of cardiovascular disease
  • Presence of metabolic disease
  • Taking regular oral medication
  • Current smoker

Treatment and study plan

BFR Exercise Training

Other

Participants will complete exercise training on a recumbent bike placed on a 45 degree incline (BFR).

CTL Exercise Training

Other

Participants will complete exercise training on a recumbent bike placed on flat ground (CTL).

Primary outcomes

  1. Citrate Synthase

    Time frame: 3 days prior to start of exercise training and 3 days after completing exercise training

    Fold change in citrate synthase maximal activity

Secondary outcomes

  1. Transcription factor A, mitochondrial (TFAM)

    Time frame: 3 days before training to 3 days after training

    Fold change in TFAM protein

  2. Nuclear respiratory factor, 1 (NRF1)

    Time frame: 3 days before training to 3 days after training

    Fold change in NRF-1 protein

  3. Maximal oxygen consumption (VO2peak)

    Time frame: 3 days before training to 3 days after training

    Change in VO2peak (POST-PRE)

  4. Heart rate (HR)

    Time frame: Every 5 minutes during each of the 7, 30-minute exercise sessions

    Average HR

  5. Revolutions per minute (RPM)

    Time frame: Every 5 minutes during each of the 7, 30-minute exercise sessions

    Average RPM

  6. Blood [lactate]

    Time frame: During all exercise sessions at 10 and 30 minutes

    Average blood lactate concentration

  7. Rating of perceived exertion (RPE)

    Time frame: Every 5 minutes during each of the 7, 30-minute exercise sessions

    Average RPE

  8. Nuclear Factor, Erythroid 2 Like 2 (NFE2L2)

    Time frame: 3 days before training to 3 days after training

    Fold change in NFE2L2

  9. Cytochrome c oxidase (COX)

    Time frame: 3 days before training to 3 days after training

    Fold change in COX

  10. Electromyography (EMG)

    Time frame: Throughout each of the 7, 30-minute exercise sessions

    Median frequency and average amplitude of EMG signal

  11. Near-infrared spectroscopy (NIRS)

    Time frame: Throughout each of the 7, 30-minute exercise sessions

    Deoxygenated hemoglobin and oxygenated hemoglobin

Sponsors and collaborators

Lead sponsor

Brendon Gurd, PhD

Other

Registry information

Official study title

Examining The Effect of Blood Flow Restricted Aerobic Training on Citrate Synthase Activity in Young Healthy Adults

Important dates

Study start
2019
Primary completion
2019
Study completion
2019
First posted
Nov 5, 2019
Registry last updated
Dec 26, 2023

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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