Evaluation of Wnt5a and LRP5 Levels in Serum and Saliva Samples and the Relationship Between Clinical Periodontal Parameters

Objectives: Wnt5a is a secreted Wnt ligand that plays an important role in cellular homeostasis. Wnt interaction with coreceptor low-density lipoprotein receptor-related protein 5 (LRP5) induces the proliferation of osteoblasts and prolongs their functional life. The aim of this study is evaluating the levels of Wnt5a and LRP5 in serum and saliva samples and relationship between clinical periodontal parameters.

Method: Saliva and serum samples were collected from 20 systemically healthy patients with stage III periodontitis and 20 periodontally healthy controls. Wnt5a and LRP5 levels were measured using enzyme-linked immunosorbent assay (ELISA). Clinical periodontal parameters including plaque index (PI), probing pocket depth (PPD), bleeding on probing (BOP), and clinical attachment level (CAL) were recorded.

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Key information

Conditions

Periodontal Disease Mouth Diseases Periodontal Diseases Periodontitis Periodontitis (Stage 3) Periodontitis Stage III Stomatognathic Diseases

Age range

18 year–70 year

Sex eligibility

All sexes

Study type

Observational

Primary location

Ankara University, Faculty of Dentistry

Ankara, Yenimahalle, 06500, Turkey (Türkiye)

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

>18 years old,
At least 16 permanent teeth except 3rd molars,
Individuals who do not use orthodontic appliances,
Individuals who are not pregnant or lactating,
Individuals without any systemic disease that may affect periodontal health,
Systemically healthy individuals,
Individuals who have not used anti-inflammatory and/or anti-microbial drugs in the last 6 months,
Individuals who have not received periodontal treatment in the last 1 year

Exclusion criteria

Pregnant/lactating individuals,
Individuals who have used anti-inflammatory and/or anti-microbial drugs in the last 6 months,
Individuals who have undergone periodontal treatment in the last 1 year,
Individuals with psychiatric illness,
Individuals with any oral infection,
Individuals with <16 teeth, excluding molars,
Individuals with alcohol dependence,
Individuals with active infectious disease (acute hepatitis, AIDS, tuberculosis), cancer or any systemic condition that may affect periodontal tissues,
Individuals receiving treatment with drugs known to affect periodontal tissues (phenytoin, cyclosporine A, calcium channel blockers)

Treatment and study plan

Primary outcomes

Wnt5a

Time frame: 1 month

Wnt5a is synthesized by activated antigen-presenting cells and its expression is induced by mycobacterial cell wall components and endotoxin in human antigen-presenting cells, and Wnt5a signaling is required for the overall inflammatory response of human macrophages during sepsis.The levels of Wnt5a in both serum and saliva samples were determined using commercially available ELISA kits. All assays were conducted according to the manufacturers' instructions.
LRP5

Time frame: 1 month

Low-density lipoprotein (LDL) receptor-related protein 5 (LRP5) is a WNT coreceptor in the LRP superfamily involved in a wide range of biological processes including endocytosis, cellular communication, lipid homeostasis and embryonic development. The levels of LRP5 in both serum and saliva samples were determined using commercially available ELISA kits. All assays were conducted according to the manufacturers' instructions.

Sponsors and collaborators

Lead sponsor

Ankara University

Other

Collaborators

The Scientific and Technological Research Council of Turkey

Registry information

Official study title

Expression of Wnt5a and WNT Coreceptor (LRP5) Levels in Stage III Periodontitis Patients

Important dates

Study start: 2024
Primary completion: 2024
Study completion: 2025
First posted: Apr 18, 2025
Registry last updated: Apr 18, 2025

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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Completed