Effects of Pre-dive Ketone Food Products on Latency to CNS Oxygen Toxicity (Aim 1)

Central nervous system (CNS) oxygen toxicity continues to be a risk for military divers and constrains their operations. Manifestations of this condition range from nausea, twitching, and tinnitus to seizures and unresponsiveness, and the latter may lead to death by drowning. The NAVY has a need for better methods to prevent or delay the onset of CNS oxygen toxicity (CNSOT) and to safely expand the scope of diving operations. It is the broad objective of this study to generate information that will enhance warfighter safety and performance in relevant NAVY operations by reducing the risk of CNS oxygen toxicity.

It is known that nutritional ketosis through a diet with a high fat-to-carbohydrate ratio (ketogenic diet) can reduce the frequency and severity of epileptic seizures in humans, and a recent animal study has shown that dietary ketosis also delays the onset of CNSOT. In recent years, ketone ester food products ketone esters have been made commercially available which may elevate circulating ketone levels. The investigators aim to investigate whether ketosis from commercially available ketogenic food products prior to a dive will delay the onset of CNSOT.

The first aim of this study will be to determine the effect of ketone food product ingestion on serum ketone levels, and document any relevant side effects. Post-ingestion ketone levels will be trended for 3 different ketone food product regimens in 15 total subjects. Data will be used to select the optimal ketone food product strategy to investigate in the second aim which will be registered separately.

Inclusion criteria

• Males & females between 18 and 39 years old.
• Predicted (Phase 1) VO2max ≥ 30 ml/kg/min (female) or 35 ml/kg/min (male).
• BMI ≤ 30.0 unless VO2max and baseline exercise profile is deemed appropriate for the study by the PI.

Exclusion criteria

• Prolonged QTc on initial ECG
• Currently pregnant or attempting to become pregnant.
• Have a medical history of:

• Smoking history deemed significant by PI
• Known significant electrolyte disorders
• Coronary artery disease
• Cardiac arrhythmia deemed significant by PI
• Lung disease
• Hypertension
• Seizures
• Exercise intolerance or inability to meet inclusion requirements
• Psychiatric disorder deemed significant by PI
• Previous pneumothorax or pneumomediastinum
• Hypo- or hyperglycemia
• Diabetes
• Inability to equalize middle ear spaces during hyperbaric compression
• Claustrophobia
• Regularly take any medications which may alter heart rate, blood pressure, neurotransmitter function, alter seizure threshold, mood or affect per PI discretion.
• Any other condition limiting ability to perform exercise testing or dive profile as determined by the investigators.