Skip to main content
OpenTrials
Completed

NCT Number: NCT07719686

Effect of Menstrual Cycle Phase on Pain Perception and Anxiety During Non-Surgical Periodontal Treatment

This study evaluates the effect of menstrual cycle phases on pain perception and dental anxiety during non-surgical periodontal therapy in premenopausal women with Stage II and III periodontitis. Pain and anxiety levels were assessed using VAS and DAS scales in perimenstrual and postmenstrual phases. Periodontal treatment outcomes were also recorded before and after therapy.

Completed

Looking for future studies?

Notify Me

Key information

Conditions

Periodontitis Mouth Diseases Periodontal Diseases Stomatognathic Diseases

Sex eligibility

Female

Study type

Observational

Primary location

Karadeniz Technical University Faculty of Dentistry, Department of Periodontology

Trabzon, 61080, Turkey (Türkiye)

About this study

This study investigated the influence of menstrual cycle phases on pain perception and anxiety levels during non-surgical periodontal therapy (NSPT) in systemically healthy premenopausal women diagnosed with Stage II and III periodontitis. A total of 36 participants were included. Clinical periodontal parameters, including probing pocket depth (PPD), clinical attachment level (CAL), gingival index (GI), plaque index (PI), and bleeding on probing (BoP), were recorded before and after NSPT. Pain perception was assessed using the Visual Analog Scale (VAS), and dental anxiety was evaluated using the Corah Dental Anxiety Scale (DAS). NSPT was performed without local anesthesia using a split-mouth design. Outcomes were compared between perimenstrual and postmenstrual phases.

Who can participate

Healthy volunteers accepted: No

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Female patients aged 18 years and older diagnosed with Stage II and III periodontitis, systemically healthy, and not using medications affecting pain perception or hormonal status. Participants with regular menstrual cycles were included.

Exclusion criteria

  • Patients with systemic diseases, pregnancy or lactation, use of analgesic, anti-inflammatory or hormonal medications, smokers (if excluded), and those with irregular menstrual cycles were excluded.

Treatment and study plan

Non Surgical Periodontal Treatment

Procedure

Standard non-surgical periodontal therapy including scaling and root planing performed without local anesthesia.

Primary outcomes

  1. Pain perception

    Time frame: Immediately after completion of each treatment session (session duration was not fixed and varied according to the extent of treatment required)

    Pain perception during non-surgical periodontal therapy assessed using the Visual Analog Scale (VAS; score range: 0-10), where higher scores indicate greater pain intensity, across menstrual cycle phases.

  2. Dental Anxiety

    Time frame: Immediately after completion of each treatment session (session duration was not fixed and varied according to the extent of treatment required)

    Dental anxiety during non-surgical periodontal therapy was assessed using the Corah Dental Anxiety Scale (DAS; score range: 4-20). The scale consists of four questions related to different dental treatment situations, each with five response options of increasing intensity. Participants were asked to select the response that best reflected their level of anxiety. Higher scores indicate greater anxiety levels, across menstrual cycle phases.

Secondary outcomes

  1. Probing Pocket Depth (PPD)

    Time frame: Baseline (pre-treatment) and 6 weeks after completion of non-surgical periodontal therapy

    PPD was measured in millimeters at six sites per tooth using a periodontal probe, and mean values were calculated per participant.

  2. Clinical Attachment Level (CAL)

    Time frame: Baseline (pre-treatment) and 6 weeks after completion of non-surgical periodontal therapy

    CAL was measured in millimeters at six sites per tooth using a periodontal probe, and mean values were calculated per participant.

  3. Gingival Index (GI)

    Time frame: Baseline (pre-treatment) and 6 weeks after completion of non-surgical periodontal therapy

    GI was assessed at four sites per tooth to evaluate gingival inflammation, and mean scores were calculated per participant.

  4. Plaque Index (PI)

    Time frame: Baseline (pre-treatment) and 6 weeks after completion of non-surgical periodontal therapy

    PI was assessed at four sites per tooth to evaluate plaque accumulation, and mean scores were calculated per participant.

  5. Bleeding on Probing (BoP)

    Time frame: Baseline (pre-treatment) and 6 weeks after completion of non-surgical periodontal therapy

    BoP was recorded as the percentage of sites showing bleeding within 10 seconds after probing.

Sponsors and collaborators

Lead sponsor

Karadeniz Technical University

Other

Registry information

Official study title

Comparison of Pain Perception and Anxiety Levels During Non-Surgical Periodontal Treatment in Female Patients With Stage II and III Periodontitis at Different Phases of the Menstrual Cycle

Acronym: MAPP

Important dates

Study start
2024
Primary completion
2025
Study completion
2025
First posted
Jul 22, 2026
Registry last updated
Jul 22, 2026

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

Related clinical trials

Published trials that share one or more normalized conditions with this study.