Description of the Endothelial Phenotypes From the Subcutaneous Abdominal and Gluteo-femoral Adipose Tissues in Women

The adipose tissues (gluteo-femoral and abdominal) will be obtained from women programmed for surgery in which the estrogenic status as well as adipose tissue mass profile will be evaluated. The phenotype (inflammatory and angiogenic activation and senescent state) of native endothelial cells will be determined "in situ" by immunohistochemical and flow cytometry approaches and on isolated endothelial cells by Reverse transcriptase-Q Polymerase Chain Reaction and Western blot. In vitro approaches will be performed 1) to establish a causal relationship between endothelial senescence and activation state, 2) to define the impact of the adipose tissue microenvironment on the endothelial cell activation and senescence and 3) to study the potential protective effect of estrogens on endothelial senescence.

Inclusion criteria

• Stable weight for at least 3 months
• Women undergoing a surgery of subcutaneous adipose tissue

Exclusion criteria

• Undergoing Insulin therapy
• Undergoing steroidal and nonsteroidal anti-inflammatory treatments
• undergoing or stopped since less than 6 months immunosuppressive treatments
• Positive serology for HIV, Hepatitis B Virus and/or Hepatitis C Virus