Metabolic Unit der Universität Hohenheim
Stuttgart, Baden-Wurttemberg, 70599, Germany
NCT Number: NCT06645756
After eating, blood composition changes, including increased triglycerides and glucose, which can trigger postprandial inflammation. Particularly with high-fat foods, pro-inflammatory lipopolysaccharide (LPS) increases. This activates leukocytes to release pro-inflammatory cytokines. In industrialized countries where "snacking" is common, many people spend the day in a postprandial state. Obese individuals tend to have chronic inflammation and show increased susceptibility to infections such as SARS-CoV-2. The main objective of the study is to investigate the response of leukocytes and the serum metabolome after food intake in individuals with obesity compared to healthy individuals, focusing on LPS as a key stimulus of innate immunity.
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Notify Me25 year and older
All sexes
Interventional
Not applicable
Stuttgart, Baden-Wurttemberg, 70599, Germany
Healthy volunteers accepted: Yes
Only the study team can determine whether someone qualifies for participation.
Inclusion criteria
Exclusion criteria
Test meal consisting of fries, chicken nuggets and eggs
Time frame: 30 min after test meal
LPS in Serum
Time frame: 60 min after test meal
LPS in Serum
Time frame: 120 min after test meal
LPS in Serum
Time frame: 180 min after test meal
LPS in Serum
Time frame: 240 min after test meal
LPS in Serum
Time frame: immediately after the intervention
To analyze the reactivity of PBMCs, these are isolated, co-cultivated with bacteria and then the number of replicable bacteria is counted.
Time frame: immediately after the intervention
Time frame: immediately after the intervention
The PBMCs are characterized by fluorescence-activated cell sorting (FACS) and RNA isolation.
Time frame: immediately after the intervention
Time frame: immediately after the intervention
Time frame: immediately after the intervention
Time frame: immediately after the intervention
Time frame: immediately after the intervention
Time frame: baseline
Time frame: immediately after the intervention
Time frame: baseline
University of Hohenheim
Other
Acronym: CAPE
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