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Completed

NCT Number: NCT05230303

Caffeine Supplementation, Resistance Training and Blood Antioxidant Status

The purpose of this study is to verify the effect of acute caffeine intake on resistance training volume and blood antioxidant status, in a randomised, double-blind, placebo-controlled crossover trial.

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Key information

Conditions

Caffeine

Age range

18 year–45 year

Sex eligibility

Male

Study type

Interventional

Phase

Not applicable

Primary location

The Jerzy Kukuczka Academy of Physical Education

Katowice, 40-065, Poland

About this study

Caffeine is one of the most commonly consumed psychoactive substances in the world. Several previous studies confirmed that caffeine improves several aspects of exercise performance, including resistance training outcomes. Interestingly, caffeine and its catabolic products, theobromine and xanthine, exhibit antioxidant and prooxidant properties. Additionally, resistance training promotes the generation of reactive oxygen species, which are important for the cellular adaptation process.

Hovewer, to the best of our knowledge, there is no information as to how caffeine combined with resistance training affects blood antioxidant status. Therefore, the aim of this study was to examine acute effects of caffeine supplementation on resistance training volume and blood antioxidant status in resistance trained men.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • written consent to participate,
  • medical permission by physician
  • "resistance-trained," defined as having a minimum of two years of resistance training experience
  • minimum of 2 workout sessions per week in the last 6 months

Exclusion criteria

  • neuromuscular or musculoskeletal disorders
  • current injury
  • using any medications, dietary supplements or ergogenic aids which could potentially affect the study outcomes
  • a positive smoking status
  • potential allergy to caffeine

Treatment and study plan

Caffeine supplementation

Dietary Supplement

Two identical experimental sessions with a one-week interval between sessions (to allow complete recovery and ensure substance wash-out) will be taken. During the experimental sessions, participants will ingest caffeine in dose of 3 mg/kg of body mass. Caffeine will be provided in capsules containing the individual amount of caffeine and will be administered orally 60 min before the onset of the exercise protocol.

Placebo treatment

Dietary Supplement

The experimental procedure for each particpant will include placebo supplementation. The manufacturer of the caffeine capsules will also prepare identical placebo capsules filled out with a microcrystalline cellulose. Placebo will be administered orally 60 min before the onset of the exercise protocol.

Primary outcomes

  1. Change the levels of oxidative stress markers

    Time frame: Day 1, Day 2

    Serum will be obtained for TOS/TOC (total oxidative stress), MDA (malondialdehyde), oncentration of TAS (total antioxidant status), activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx); concentartion of GSH (reduced glutathione) and UA (uric acid)

  2. Changes in markers of muscle damage

    Time frame: Day 1, Day 2

    Serum will be obtained for activity of creatine kinase (CK) and lactate dehydrogenase (LDH), concentration of myoglobin (Mb)

  3. Changes in Inflammation markers

    Time frame: Day 1, Day 2

    Serum will be obtained for level of interleukin 6, TNF alfa (tumor matrix factor), CRP (C-reactive protein)

  4. Changes in markers of liver function

    Time frame: Day 1, Day 2

    Serum will be obtained for activity of ASPAT (aspartate aminotransferase), ALAT (alanine aminotransferase) and gamma glutamyltransferase (GGT)

Secondary outcomes

  1. Changes in resistance training volume

    Time frame: During caffeine and placebo treatment

  2. Changes in rate of perceived exertion after resistance training

    Time frame: Immediately after exercise protocol during caffeine and placebo treatment

  3. Changes in heart rate during resistance training

    Time frame: During caffeine and placebo treatment

  4. Changes in muscle soreness

    Time frame: prior to exercise, and 24, 48, 72, 96 and 120 hours post exercise.

Sponsors and collaborators

Lead sponsor

The Jerzy Kukuczka Academy of Physical Education in Katowice

Other

Registry information

Official study title

Acute Effects of Caffeine Supplementation on Resistance Training Volume and Blood Antioxidant Status in Resitance Trained Men

Important dates

Study start
2022
Primary completion
2023
Study completion
2023
First posted
Feb 8, 2022
Registry last updated
Mar 10, 2023

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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