Arginine Feeding: a Novel Strategy to Improve Protein Metabolism in Cancer and the Response to Surgery

In the present proposal, the effects of surgery and cancer will be examined by comparing subjects undergoing breast surgery because of malignancy vs. prophylactic reasons (aim 1). Furthermore, the effects of one-week pre-operative protein feeding with or without enrichment with arginine on post-operative protein metabolism (aim 2) will be investigated in the cancer group. Variables of interest are: 1. Whole-body and skeletal muscle protein metabolism, whole body arginine turnover and de-novo arginine production rate, and the anabolic capacity to feeding(assessed by stable isotope methodology). 2. Body weight, muscle mass and functional status, score for well-being (assessed by Profile of Mood State and Mini Mental State).

In the present study, we propose that a nutritional supplement that is high in protein content and enhanced in arginine will be more effective than a typical commercial nutritional supplement in diminishing the catabolic effects of surgery in subjects with cancer, thereby optimizing their quality of life. If this is found to be the case, this would provide the basis for reformulating the nutritional composition in accord with the effects of cancer and surgery on protein metabolism.

Inclusion criteria

Cancer groups (for aims 1 and 2)

• Recently diagnosed (up to 4 weeks prior to treatment for cancer) with stage I, II or III invasive breast cancer
• Undergoing mastectomy
• Age greater than 30 years
• Ability to sign informed consent
• Good performance status defined by ECOG scale 0,1 or 2 (see CRF performance status)

Control group (for aim 1)

• Age greater than 30 years
• Undergoing prophylactic mastectomy
• Ability to sign informed consent
• Good performance status defined by ECOG scale 0,1 or 2 (see CRF performance status)

Exclusion criteria

All groups (aim 1 and 2)

• Body weight loss of greater than 10% in the past 3 months
• Previous anti-cancer therapy (e.g. chemotherapy or radiotherapy) or surgery less than 4 weeks prior to the experiment
• Diagnosed diabetes type I or II
• Untreated metabolic diseases including liver or renal disease
• Any documented autoimmune disease
• Use of corticosteroids, beta-antagonists or nitrovasodilators
• Use of supplements enriched with amino acids
• Presence of acute illness or metabolically unstable chronic illness
• Unstable heart disease requiring therapy or recent myocardial infarction (less than 1 year)
• Current alcohol or drug abuse (ETOH more than 2 servings per day)
• Allergy/intolerance to any of the ingredients of the study products
• Any other condition deemed by the PI and the study physician as exclusion or that interferes with proper conduct of the study/ safety of the patient.