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NCT Number: NCT06542042

Application of Nanopore Adaptive Sequencing

Infection post liver transplantation is an important factor in the death in patients. The traditional method of diagnosing infection post liver transplantation is laboratory tests. But the sensitivity and specificity of blood tests is poor. Next-generation sequencing (NGS) has greater detection rate for mycobacterium tuberculosis, anaerobes and fungi and greater sensitivity compare with blood tests. However use of NGS is limited because of the short read-length. Oxford nanopore adaptive sequencing (NAS) method is the Third Revolution in Sequencing Technology. For each 1 Gbp of data, NAS sequencing detected 45 times more microbiome sequences than Nanopore standard sequencing and 2.5 times more than Illumina sequencing. The purpose of this study is to compare NAS with NGS and laboratory tests for the diagnostic rate of infection post liver transplantation.

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Key information

Conditions

Liver Transplant Infection

Sex eligibility

All sexes

Study type

Observational

Primary location

About this study

Based on the previous work, the investigators found that: 1. NAS has a higher microbial enrichment efficiency and can detect pathogenic information more quickly compared with nanopores normal sequencing (NNS); 2. NAS can detect more specific pathogen fragments with short-time sequencing; 3. NAS can cover more pathogenic genomes than NNS in a short-time; 4. NAS can detect antibiotic resistance information of pathogenic bacteria in clinical samples.

Who can participate

Healthy volunteers accepted: No

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Accept liver transplantation
  • Signing informed consent voluntarily
  • Possessing ability to comprehend material information
  • Participating this study voluntarily

Exclusion criteria

  • Participated another study
  • Graft loss
  • Have undergone a multi-organ transplantion or have had a previous organ transplantation
  • Patient died

Treatment and study plan

NAS, NGS and Laboratory tests

Diagnostic Test

Using three diffrent detection methods and comparing their efficiency

Primary outcomes

  1. Diagnosed infection post liver transplantation

    Time frame: Post liver transplantation before patients discharge from hospital, an average of 1 months.

    Any pathogens are detected in alveolar lavage fluid, peritoneal drainage or blood before patients discharge from hospital, an average of 1 months

Study contacts

Contact information is provided by the study sponsor or research team.

pusen Wang, Dr

CONTACT

[email protected]

18621006138

Sponsors and collaborators

Lead sponsor

Shenzhen Third People's Hospital

Other

Registry information

Official study title

Application of Oxford Nanopore Adaptive Sequencing Method in Infection Post Liver Transplantation

Important dates

Study start
2024
Primary completion
2026
Study completion
2026
First posted
Aug 7, 2024
Registry last updated
Aug 7, 2024

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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