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NCT Number: NCT07148908

Animal and Microbial-based Dietary Protein Efficiency in Adults

Given the relatively high carbon footprint and sustainability of animal-based proteins, there is a growing interest in determining the nutritional quality non-animal-based protein sources. The overall objective of this investigation is to examine the impact of different animal and microbial-based protein sources to support whole body protein synthesis in adults. To do this, investigators will employ a 'breath test' method developed in our laboratory as well as urine sampling. The results of this study will allow us to better understand the impact of dietary protein quality for maintaining health and body protein mass in adults.

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Key information

Conditions

Age range

18 year–45 year

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

Primary location

Kinesiology & Physical Education

Toronto, Ontario, M5S 2C9, Canada

Location status: Recruiting

Location contact

Sabrina T. Barsky, Ph.D.

CONTACT

[email protected]

647-540-0115

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Healthy, male and female, recreationally-active participants.
  • Healthy will be defined as screened by the PAR-Q+ (Appendix B) and The Physical Activity Readiness Questionnaire (Appendix C).
  • Participants will be aged 18-45 years old.
  • Participants are willing to abide by the compliance rules of this study.
  • Self-reported regular menstrual cycle (25-35d) within the last 3 months (female participants).
  • Use of monophasic combined oral contraceptives (COC) containing 21 active pills (e.g., Yaz, Marvelon, Cyclen, etc.; the brand of COC will be recorded) (female participants).

Exclusion criteria

  • Inability to adhere to any of the compliance rules judged by principal investigator (e.g. dairy protein or yeast allergy).
  • Self-reported regular tobacco use.
  • Self-reported illicit drug use (e.g., growth hormone, testosterone, etc.).
  • Individuals who have participated in studies within the past year involving any of the stable isotopes in the study.
  • Use of multiphasic COCs due to the difficulty of controlling for hormonal fluctuations in such formulations (female participants).
  • Use of COCs containing >21 active pills per cycle due to potential differences in their effect on metabolism (female participants).
  • Use of other forms of hormonal contraception (e.g., progestin-only pill, hormonal IUD, intravaginal ring) (female participants).
  • Not currently or previously (in the past 6 months) on a vegan diet

Treatment and study plan

Yeast Protein Supplement

Dietary Supplement

Each protein will be consumed at 0.9g/kg/d in n=8 hourly drinks

Whey Protein Isolate Supplement

Dietary Supplement

Each protein will be consumed at 0.9g/kg/d in n=8 hourly drinks

Collagen Hydrolysate Supplement

Dietary Supplement

Each protein will be consumed at 0.9g/kg/d in n=8 hourly drinks

Primary outcomes

  1. 1. Phenylalanine excretion (umol/kg/h)

    Time frame: 8 hours

    Whole body phenylalanine excretion determined from the product of breath 13CO2 enrichment and carbon dioxide production rate (VCO2).

  2. Phenylalanine oxidation (umol/kg/h)

    Time frame: 8 hours

    Whole-body phenylalanine oxidation determined from the correction of phenylalanine excretion by the phenylalanine precursor enrichment determined from urine enrichment over the 8 hour measurement period.

Study contacts

Contact information is provided by the study sponsor or research team.

Daniel R. Moore, Ph.D.

CONTACT

[email protected]

416-946-4088

Sponsors and collaborators

Lead sponsor

Daniel Moore

Other

Collaborators

  • Lesaffre International

Registry information

Official study title

The Effects of Animal- and Microbial-based Protein Sources on Whole-body Protein Metabolism in Healthy Adults

Acronym: YPQ

Important dates

Study start
2025
Primary completion
2025
Study completion
2025
First posted
Aug 29, 2025
Registry last updated
Aug 29, 2025

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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