Allogeneic γδT Cells in Glioblastoma

Inclusion criteria

• Male or female, age 18-70 years old (both ends included)
• At least one evaluable lesion with previous biopsy or pathohistologic confirmation of glioblastoma (WHO grade IV), with imaging suggestive of continued progression or recurrence after comprehensive treatment
• Karnofsky Performance Status (KPS) ≥ 60%
• Life expectancy > 4 weeks
• Patients who completed radiotherapy or systemic therapies (including temozolomide/bevacizumab or other agents) for at least 4 weeks prior to enrollment. All prior treatment-related toxicities should be defined as ≤ grade 1 (except for toxicities such as alopecia or leukoplakia) according to the Common Terminology Standard for Adverse Events (CTCAE 6.0)
• Must be able to undergo an MRI with contrast
• Must have adequate organ and marrow function as defined below:

• White blood cell count (WBC) ≥ 3 x 10^9/L
• Absolute neutrophil count (ANC) > 1 x 10^9/L
• Hemoglobin (Hb) ≥ 90 g/L
• Platelet (PLT) ≥ 80×10^9/L
• Albumin transaminase (ALT) & albumin transaminase (AST) < 1.5 × institutional upper limit of normal (ULN)
• Serum creatinine (Cr) < 1.5 x institutional ULN
• Total bilirubin < 1.5 x institutional ULN
• PT & PTT ≤ 1.25 x institutional ULN
• No obvious hereditary diseases
• Normal cardiac function with left ventricular ejection fraction >55%
• No bleeding and coagulation disorders
• Absence of positive blood cultures for bacteria, fungus, or virus within 48-hours prior to ABOUT γδT cell infusion and/or there aren't any indications of meningitis
• Fertile women must have had a pregnancy test with a negative result within 7 days prior to the start of treatment, and subjects are willing to use contraception (hormonal or barrier method of birth control or abstinence) during the clinical trial and for 6 months after the last cell infusion; should a woman become pregnant or suspect that she is pregnant while participating on the trial, she should inform her treating physician immediately
• Signed, written informed consent

Exclusion criteria

• Active hepatitis B or C virus, HIV infection, or other untreated active infection
• Pregnant and lactating women
• Participants with organ failure
• Participants with a chronic disease requiring immunologic or hormonal therapy
• Participants with an allergy to immunotherapy and related cells
• Participants with uncontrolled intercurrent illness
• Participants with psychiatric illness/social situations that would limit compliance with study requirements
• Participants with a history of organ transplantation or who are awaiting organ transplantation