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NCT Number: NCT07826598

Blood Parameter Changes Following Aerobic Exercise in Professional Soccer Players

This study aims to investigate acute changes in serum ferritin, folate, vitamin B12, and parathyroid hormone (PTH) levels following aerobic exercise in professional soccer players. Twenty professional male soccer players will voluntarily participate in the study. Participants will complete a progressive 20-meter shuttle run test. Venous blood samples will be collected immediately before and after the exercise protocol. Pre- to post-exercise changes in each blood parameter will be analyzed to determine the acute physiological responses to aerobic exercise in professional soccer players.

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Key information

Conditions

Exercise Physiology Blood Parameters

Age range

18 year–25 year

Sex eligibility

Male

Study type

Interventional

Phase

Not applicable

Primary location

Exercise Physiology Laboratory, Faculty of Sport Sciences, Karabuk University

Karabük, Turkey (Türkiye)

About this study

This single-group interventional study is designed to examine acute changes in selected blood biomarkers following aerobic exercise in professional soccer players. A total of 20 professional male soccer players aged 18-25 years will voluntarily participate. Participants will be recruited from a professional soccer club in Karabuk, Türkiye. Before participation, all individuals will receive information about the study procedures, provide written informed consent, and undergo medical screening to determine their eligibility for maximal aerobic exercise.

Body height and body mass will be measured before exercise testing. Aerobic exercise will be performed using a progressive 20-meter shuttle run test. Participants will run continuously between two lines positioned 20 meters apart while following prerecorded audio signals. Running speed will progressively increase as the interval between the audio signals decreases. The test will end when a participant fails to reach the designated line before the audio signal on two consecutive occasions.

Venous blood samples of approximately 5 mL will be collected immediately before and immediately after the exercise protocol by qualified healthcare personnel. Blood samples will be collected into anticoagulant-free serum tubes. Following a clotting period of approximately 30 minutes, the samples will be centrifuged at 3,000 revolutions per minute to separate the serum. Serum samples will be stored at -70°C until laboratory analysis.

Serum ferritin, folate, vitamin B12, and parathyroid hormone (PTH) concentrations will be measured. Pre- to post-exercise changes in each biomarker will be analyzed separately to determine the acute physiological responses elicited by aerobic exercise in professional soccer players.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

- Professional soccer players.

  • Willing and able to participate in the study procedures.
  • Provision of informed consent.

Exclusion criteria

- Inability to complete the aerobic exercise protocol.

  • Withdrawal from the study or unwillingness to continue participation.

Treatment and study plan

20-Meter Shuttle Run Test

Behavioral

Participants will perform a progressive 20-meter shuttle run test by running continuously between two lines positioned 20 meters apart in time with prerecorded audio signals. Running speed will progressively increase as the interval between the audio signals decreases. The test will end when the participant fails to reach the designated line before the signal on two consecutive occasions.

Primary outcomes

  1. Change in Serum Ferritin Concentration From Pre-Exercise to Immediately Post-Exercise

    Time frame: Immediately before exercise and immediately after completion of the 20-meter shuttle run test

    Venous blood samples will be collected immediately before and immediately after the 20-meter shuttle run test. Serum ferritin concentration will be measured in ng/mL. For each participant, the change will be calculated by subtracting the pre-exercise value from the post-exercise value. The group-level outcome will be summarized using the mean change and standard deviation.

  2. Change in Serum Folate Concentration From Pre-Exercise to Immediately Post-Exercise

    Time frame: Immediately before exercise and immediately after completion of the 20-meter shuttle run test

    Venous blood samples will be collected immediately before and immediately after the 20-meter shuttle run test. Serum folate concentration will be measured in ng/mL. For each participant, the change will be calculated by subtracting the pre-exercise value from the post-exercise value. The group-level outcome will be summarized using the mean change and standard deviation.

  3. Change in Serum Vitamin B12 Concentration From Pre-Exercise to Immediately Post-Exercise

    Time frame: Immediately before exercise and immediately after completion of the 20-meter shuttle run test

    Venous blood samples will be collected immediately before and immediately after the 20-meter shuttle run test. Serum vitamin B12 concentration will be measured in pg/mL. For each participant, the change will be calculated by subtracting the pre-exercise value from the post-exercise value. The group-level outcome will be summarized using the mean change and standard deviation.

  4. Change in Serum Parathyroid Hormone Concentration From Pre-Exercise to Immediately Post-Exercise

    Time frame: Immediately before exercise and immediately after completion of the 20-meter shuttle run test

    Venous blood samples will be collected immediately before and immediately after the 20-meter shuttle run test. Serum parathyroid hormone (PTH) concentration will be measured in pg/mL. For each participant, the change will be calculated by subtracting the pre-exercise value from the post-exercise value. The group-level outcome will be summarized using the mean change and standard deviation.

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Sponsors and collaborators

Lead sponsor

Inonu University

Other

Registry information

Official study title

Aerobic Exercise-Induced Changes in Ferritin, Folate, Vitamin B12, and Parathyroid Hormone Levels in Professional Soccer Players

Acronym: ABP-Soccer

Important dates

Study start
2026
Primary completion
2026
Study completion
2026
First posted
Sep 17, 2026
Registry last updated
Sep 17, 2026

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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