Weight Cut Stress: the Hidden Struggle Beyond Fight Day

Objective:

This study aims to investigate the effects of rapid weight-cutting on objective (Heart Rate Variability - HRV, measured via RMSSD) and subjective (Visual Analog Scale - VAS) stress parameters in elite kickboxers. The evaluation will occur across seven specific time points: Weigh-in Morning, Pre-Weigh-in, Post-Weigh-in, Post-Weigh-in Meal, Match Day Morning, Pre-Match, and Post-Match.

Study Design:

An observational comparative design will be employed to assess objective and subjective stress responses related to weight-cutting in elite kickboxers. The study will take place during the Senior European Kickboxing Championship in Greece, from November 2 to November 10, 2024, under real competition conditions. Two groups will be included: a weight-cutting group and a non-weight-cutting group. This design will enable comparisons between objective (RMSSD) and subjective (VAS) stress measures, as well as between athletes who reduce weight and those who do not. Data will be collected at seven key time points to capture stress variations during the weight-cutting and competition phases.

Participants:

Elite kickboxers will be selected based on specific inclusion criteria. A sample size calculation using G*Power, based on an ANOVA with repeated measures and within-between interaction, determined a minimum of 22 participants to ensure adequate statistical power. Parameters include an effect size of 0.25, an alpha level of 0.05, and a power of 0.90. Accounting for potential data loss, the study targets 25 participants, with 12 in the weight-cutting group and 13 in the non-weight-cutting group. Participants will be active male elite kickboxers, aged 18-35, with at least five years of national or international competitive experience. The weight-cutting group will consist of athletes losing at least 5% of their body weight within 48 hours before the weigh-in, while the non-weight-cutting group will include those with no weight-cutting procedures and a maximum weight loss of 2% during pre-weigh-in training. Participation will be voluntary, with athletes provided detailed information about the study's purpose, methods, and risks, and written informed consent will be obtained. Confidentiality will be ensured, and data will be used solely for research purposes. The study has been approved by the Erzurum Technical University Scientific Research and Publication Ethics Committee (Meeting No: 10, Decision No: 10, Date: 12.09.2024).

Data Collection:

Data collection will focus on objective and subjective stress parameters using HRV and VAS. Objective stress will be measured via RMSSD values, recorded with Apple Watch Series 8 devices equipped with photoplethysmography technology. Participants will wear the device in a calm, seated position for five-minute intervals to ensure reliable HRV data. Subjective stress will be assessed using the VAS, where participants rate their perceived stress on a scale. Measurements will occur at seven time points surrounding the weigh-in and competition period: Weigh-in Morning, Pre-Weigh-in, Post-Weigh-in, Post-Weigh-in Meal, Match Day Morning, Pre-Match, and Post-Match.

Data Analysis:

Data will be analyzed using SPSS version 26. Pairwise group comparisons will use Hedges' g effect size calculations, adjusted for small sample sizes, and interpreted classification (trivial < 0.2, small 0.2-0.59, moderate 0.60-1.19, large 1.20-1.99, very large 2.0-3.99, nearly perfect > 4.0). Changes across the seven time points for RMSSD and VAS will be examined with repeated measures ANOVA, including within-between interactions. Significant effects or interactions will be followed by Bonferroni-adjusted post hoc tests to control for multiple comparisons. Partial eta-squared (η²ₚ) values will be reported to evaluate practical significance. Statistical significance will be set at p < 0.05.

Inclusion criteria

• Elite male kickboxers aged between 18 and 35 years
• Minimum 5 years of competitive experience at the national or international level
• Actively competing in the Senior European Kickboxing Championship
• For the weight-cutting group: Must have lost at least 5% of body weight within the 48 hours prior to weigh-in

Exclusion criteria

• Athletes who lost between 3-4% of their body weight, as they do not fit into either group
• History of cardiovascular diseases
• History of metabolic disorders
• Any medical condition that could affect HRV measurements
• Current or recent injuries (within the last 3 months) that could impair performance or stress responses