Use of the Ozone in Periodontal Treatment

The study has been designed in a randomized parallelly-controlled and double-blinded design as a full-mouth clinical trial. The duration of the study was 1 month.

The study was performed with two groups; The Test Group, and the Control Group. SRP plus gaseous ozone [1 W (100 mJ, 10 Hz), (n=20)] was applied to the Test Group.

The Control Group, on the other hand, had SRP plus placebo (n=20). The patients were distributed in random order to the 2 groups (each group had 20 patients). One patient in the Ozone Group and 2 patients in the Control Group left the study.

The following clinical periodontal parameters were performed immediately before SRP (baseline), and 1 month after treatment for each test and control groups. Plaque index, GI, PD and CAL were recorded. All clinical parameter measurements were used a manual periodontal probe (PCP- 12, Hu-Friedy, Chicago, IL, USA).

The total antioxidant status, total oxidant status, nitric oxide, 8-hidroxi-deoksiguanosine, myeloperoxidase, glutathione, malondialdehyde and transforming growth factor-beta levels were determined in the saliva samples.

Inclusion criteria

• patients having had generalize CP which were agreed upon by consensus at the World Workshop in Periodontics in 1999 [24];
• three or more teeth having at least two quadrants with probing depth between 4 and 6 mm and radiographic signs of bone loss;
• aged 30 years and above;
• a minimum of 20 teeth.

Exclusion criteria

• received periodontal therapy within the last 12 months;
• systemic diseases which could affect periodontal treatment outcomes;
• having taken systemic antibiotics within the last 6 months;
• pregnancy or breast-feeding for female patients.