Use of Biopsies of Abdominal Subcutaneous Fat to Study the Role of the Activation of Endocannabinoid 1 Receptors (CB1R) on Adipocyte Glucolipid Metabolism

Obesity is associated with hyperactivation of the endocannabinoid system, and its inhibition by the administration of CB1 receptor (CB1R) antagonists, leads to a decrease in food intake, weight loss and an improvement in metabolic parameters. Even though the reduction in food intake following central CB1R inactivation seems to be the principal cause of weight loss and the improvement in metabolic parameters, several studies in animals and humans have indicated that peripheral CB1R could also be implicated in the regulation of glucolipid metabolism. As a result, it has been suggested that the long-term beneficial effects of inactivation of the endocannabinoid system are due to both central effects on food intake and peripheral effects involving adipose tissue, the liver, skeletal muscle and the pancreas. It appears essential to determine the role of CB1R located in peripheral tissues and in particular in adipose tissue, which plays an active role in maintaining glucolipid homeostasis. The experiments conducted in this project consist in studying in biopsies of abdominal subcutaneous fat whether activation of adipocyte CB1R modifies adipocyte metabolism by determining the mechanisms. The investigators hypothesize that activation of CB1R in adipose tissue will lead to the stimulation of lipolysis dependent on the alteration of the insulin signal, and therefore that inactivation of the endocannabinoid system by blocking peripheral CB1R could constitute a therapeutic approach to improve obesity-related insulin resistance and dyslipidaemia.

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Key information

Conditions

Hernia Surgery

Age range

18 year–70 year

Sex eligibility

Male

Study type

Observational

Primary location

CHU Dijon Bourgogne

Dijon, 21000, France

Who can participate

Healthy volunteers accepted: No

Only the study team can determine whether someone qualifies for participation.

healthy subjects

Inclusion criteria

men aged 18 to 70 years
persons who have provided oral consent
persons undergoing surgery for hernia

Exclusion criteria

persons without health insurance cover
BMI > 30
diabetes
associated diseases: cancer, chronic inflammatory diseases
adults under guardianship

obese subjects

Inclusion criteria

men aged 18 to 70 years
BMI > 30
persons who have provided oral consent
persons undergoing surgery for hernia

Exclusion criteria

persons without health insurance cover
diabetes
associated diseases: cancer, chronic inflammatory diseases
adults under guardianship

diabetic obese subjects

Inclusion criteria

men aged 18 to 70 years
type 2 diabetic not treated with Insulin or GLP-1 agonist
BMI > 30
persons who have provided oral consent
persons undergoing surgery for hernia

Exclusion criteria

persons without health insurance cover
associated diseases: cancer, chronic inflammatory diseases
adults under guardianship

Treatment and study plan

Samples of adipose tissue

Procedure

Samples of visceral and subcutaneous adipose tissue collected during hernia surgery

Primary outcomes

Glucolipid metabolism analysis in human abdominal subcutaneous and visceral fat

Time frame: Baseline

Sponsors and collaborators

Lead sponsor

Centre Hospitalier Universitaire Dijon

Other

Registry information

Acronym: CB1R

Important dates

Study start: 2016
Primary completion: 2020
Study completion: 2020
First posted: Jun 28, 2017
Registry last updated: Feb 22, 2024

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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