The Study Observes How Long Patients With Non-small Cell Lung Cancer (NSCLC) Benefit From Treatment With Epidermal Growth Factor Tyrosine Kinase Inhibitor (EGFR-TKI) When Given Either for Uncommon Mutations or for Common Mutations in the Sequence Afatinib Followed by Osimertinib

Uncommon Mutation Cohort: Time on treatment with EGFR-TKI assessed as the time from start of EGFR-TKI treatment until the end of treatment or death by any cause is reported.

Common mutation cohort: Time on treatment with EGFR-TKI assessed as the time from start of afatinib (Gi(l)otrif®) as first-line treatment until the end of the second line treatment (the last dose of osimertinib) or death date by any cause.

Time on treatment was analysed using Kaplan-Meier method, and the median was tabulated along with two-sided 95% confidence interval using the Greenwood's variance estimate.

Overall response rate (ORR) using RECIST criteria as assessed by investigator. ORR to index line Epidermal Growth Factor Receptor-Tyrosine Kinase Inhibitor (EGFR-TKI) treatment is reported (ORR is defined as number of patients with complete or partial response evaluated by Response Evaluation Criteria In Solid Tumors Criteria (RECIST 1.1)).

(Per RECIST 1.1: Complete Response (CR), Disappearance of all target lesions; Partial Response (PR), >=30% decrease in the sum of the longest diameter of target lesions; Overall Response (OR) = CR + PR).

Overall response rate (ORR) using RECIST criteria as assessed by investigator. Overall response rate to first line afatinib treatment for the Common Epidermal Growth Factor Receptor (EGFR) mutation cohort is reported. (ORR is defined as number of patients with complete or partial response evaluated by Response Evaluation Criteria In Solid Tumors Criteria (RECIST 1.1)).

(Per RECIST 1.1: Complete Response (CR), Disappearance of all target lesions; Partial Response (PR), >=30% decrease in the sum of the longest diameter of target lesions; Overall Response (OR) = CR + PR).

Overall response rate (ORR) using RECIST criteria as assessed by investigator. Overall response rate to second-line treatment (Osimertinib) is reported. (ORR is defined as number of patients with complete or partial response evaluated by Response Evaluation Criteria In Solid Tumors Criteria (RECIST 1.1)).

(Per RECIST 1.1: Complete Response (CR), Disappearance of all target lesions; Partial Response (PR), >=30% decrease in the sum of the longest diameter of target lesions; Overall Response (OR) = CR + PR).

Uncommon Mutation Cohort: Overall survival since index line treatment start of Tyrosine Kinase Inhibitor (TKI) medication administered per generation until death date by any cause or the end of index line is reported. Sequencing cohort: Overall survival for since first-line afatinib treatment start until death date by any cause or the end of index line. Kaplan-Meier estimates of quartiles of time to death were computed (with their 95% Confidence Intervals, using Greenwood's variance estimate.

Number of participants for each type of biological samples used for mutation detection at first line treatment start is reported.

The reported types of biological samples are:

• Tissue, histological sample (solid biopsy);
• Cytological sample;
• Blood (liquid biopsy);
• Other and
• Unknown.

Number of participants for each type of methodologies used for mutational testing is reported.

The reported types of methodology are:

• Amplification Refractory Mutation System (ARMS);
• Polymerase Chain reactions (PCR)-based techniques;
• Sequencing;
• Next-Generation Sequencing (NGS);
• Other;
• Unknown.

Time on treatment until failure of second-line (TTF2), defined as time elapsed from start of first-line treatment (regardless the type of treatment) to stop of second-line (regardless of the type of treatment) or death by any cause is reported. Kaplan-Meier estimates of quartiles of time to second-line treatment failure were computed (with their 95% Confidence Intervals, using Greenwood's variance estimate).

Number of participants for each type of biological samples used for mutation detection at second line treatment start is reported.

The reported types of biological samples are:

• Tissue, Histological sample (solid biopsy);
• Cytological sample;
• Blood (liquid biopsy);
• Not applicable-Clinical evaluation;
• Other;
• Unknown. Not applicable - Clinical evaluation: The uncommon Epidermal Growth Factor Receptor (EGFR) mutational status had become available after Progression on conventional second-line therapy. Erlotinib was given as state of the art second-line therapy in 2014, and an EGFR mutation was clinically suspected due to the Long-Lasting response. However, due to the unavailability of tumor tissue, this could be proven only after liquid biopsy had subsequently become available at the center in 2016.

For the Sequencing cohort second-line treatment start is initiated by the beginning of the therapy with Osimertinib.

Number of participants for each type of biological samples used for mutation detection at second line treatment stop/end of observation is reported.

The reported categories of biological samples are:

• Tissue, Histological sample (solid biopsy);
• Cytological sample;
• Blood (liquid biopsy);
• Other.

Number of participants for each type of biological samples used for mutation detection at index therapy start (index therapy refers to therapy switch from first-line chemotherapy to second-line Epidermal Growth Factor Receptor-Tyrosine Kinase Inhibitor (EGFR-TKI) therapy (index line)) is reported.

The reported categories of biological samples are:

• Tissue, Histological sample (solid biopsy);
• Cytological sample;
• Blood (liquid biopsy);
• Other and
• Unknown.

Number of participants for each type of methodology used for mutational testing at second-line treatment start is reported.

The reported types of methodologies are:

• Amplification Refractory Mutation System (ARMS);
• Polymerase Chain Reaction (PCR)-based techniques;
• Sequencing;
• Next-Generation Sequencing (NGS);
• Other;
• Unknown/Not applicable- Clinical evaluation.

Number of participants for each type of methodology used for mutational testing is reported.

The reported types of methodologies are:

• Polymerase Chain Reaction (PCR)-based techniques;
• Next-Generation Sequencing (NGS);
• Unknown.

Number of participants for each type of methodology used for mutation detection at index therapy start is reported. Index therapy refers to therapy switch from first-line chemotherapy to second-line Epidermal Growth Factor Receptor-Tyrosine Kinase Inhibitor (EGFR-TKI) therapy (index line).

The reported categories of methodology are:

• Amplification Refractory Mutation System (ARMS),
• Polymerase chain reaction based techniques (PCR-based techniques),
• Sequencing,
• Next-Generation Sequencing (NGS),
• Unknown.

Number of participants for each type of methodology used for mutation detection at start of first-line chemotherapy before index line is reported.

The reported types of methodologies are:

• PCR-based techniques;
• Sequencing;
• Next-Generation Sequencing (NGS);
• Unknown.

Index therapy refers to therapy switch from first-line chemotherapy to second-line Epidermal Growth Factor Receptor-Tyrosine Kinase Inhibitor (EGFR-TKI) therapy (index line).

Number of participants for each type of biological samples used for mutation detection at start of first-line chemotherapy before index line is reported. Index therapy refers to therapy switch from first-line chemotherapy to second-line Epidermal Growth Factor Receptor-Tyrosine Kinase Inhibitor (EGFR-TKI) therapy (index line).

The reported types of biological samples are:

• Tissue, Histological sample (solid biopsy);
• Cytological sample, Blood (liquid biopsy).