The Purple Grape Juice Study

The study treatment period will be 90 days (13 weeks, or 3 months) and the treatment dose will be 7 mL/kg/day. The treatment dose is a standard dose previously worked out in other research and was used in a variety of other clinical research (27, 32). Study randomization will be performed in a double-blind fashion with study investigators and participants unaware of group assignment. Randomization order will be created using a randomized blocked design. After volunteer consent is provided, the clinical study coordinator will open a sequentially-numbered envelope containing the study group assignment and provide a 4 week supply of study beverage.

Participants will be seen for follow-up study visits at approximately 4 week intervals after the baseline enrollment visit. Compliance with study treatment (PGJ or placebo) will be assessed by interview at visits 2, 3, and 4. At the conclusion of visits 2 and 3, a supply of study beverage will be provided to the participant for consumption during the ensuing 4 weeks. Study beverage supplies remaining at the end of the 90-day study period will be donated to each participant.

Platelet Aggregation testing will be performed by ThromboVision (Salt Lake City, UT) using multiple platelet agonists, including ADP, collagen/epinephrine, PMA, and TRAP. Each of these aggregation inducers target a separate platelet activation pathway.

Inclusion criteria

• The volunteer (male or non-pregnant female, any ethnicity) must be > 18 years of age.
• The volunteer has no history of a physician diagnosis of atherosclerosis such as carotid, peripheral, or coronary artery disease (CAD).
• The volunteer has no history of a physician diagnosis of pulmonary embolism (PE), MI, or stroke.
• The volunteer must sign a written informed consent, prior to the procedure, using a form that is approved by the local Institutional Review Board.

Exclusion criteria

• A diagnosis if diabetes mellitus.
• The limitations for specific medications, supplements and food items are exceeded as follows:

More than 1 normal dose of the following medications and/or supplements once a week during the 3 months prior to enrollment:

• aspirin • ibuprofen • fish-oil extracts
• antioxidants • vitamins

More than 1 normal serving per week in the 3 months prior to enrollment:

• other grape juices • tea • wine
• beer • alcoholic drinks • grapes

More than 5 servings per day in any combination in the 7 days (1 weeks prior to enrollment:

• non-grape juices • garlic • broccoli
• apples • any type of berries • onions Volunteer is pregnant or lactating at the time of enrollment.

Secondary Exclusions:

• Use of any of the above listed items during the 12 week course of study treatment will be documented and excess use 3 times or more will result in an administrative withdrawal of the volunteer from the study prior to the measurement of the next monthly platelet aggregation laboratory values (although those values will be measured for exploratory evaluation and the participant will remain under treatment until the end of the 12 week period).
• Routine consumption of fruit juices, or of >5 servings per day of referenced fruits or vegetables, will result in administrative withdrawal of the participant from the study's primary aim.
• Although it is unlikely the use of PGJ or look alike/ taste alike (placebo) beverage will harm the pregnant or lactating woman, the dietary restrictions placed on the participant for the duration of the study may conflict with dietary recommendations for pregnant or lactating women. Women of child bearing potential, therefore, will meet a secondary exclusion if they become pregnant.